EDN1 HEK293 Cell Transient Overexpression Lysate(Non-Denatured)
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Specification
Transfected Cell Line
HEK293
Plasmid
pCMV-EDN1 full length
Host
Human
Theoretical MW (kDa)
24
Lysis Buffer
Modified RIPA Lysis Buffer:50 mM Tris-HCl pH 7.4, 150 mM NaCl, 1mM EDTA, 1% Triton X-100, 0.1% SDS, 1% Sodium deoxycholate, 1mM PMSF.
Concentration
2 mg/ml
Quality Control Testing
Transient overexpression cell lysate was tested with Anti-EDN1 antibody (H00001906-M01) by Western Blots.
SDS-PAGE Gel
EDN1 transfected lysate
Western Blot
Lane 1: EDN1 transfected lysate ( 24 KDa).
Lane 2: Non-transfected lysate.Recommend Usage
Use it directly for immuno-precipitation, or heat lysate with SDS gel loading buffer to 95°C for 5 minutes followed by rapid cooling for western blot application. If dissociating conditions are required, add reducing agent prior to heating.
Storage Buffer
In modified RIPA Lysis Buffer.
Storage Instruction
Store at -80°C. Aliquot to avoid repeated freezing and thawing.
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Applications
Western Blot
Immunoprecipitation
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Gene Info — EDN1
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Interactome
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Pathway
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Disease
- Abortion
- Albuminuria
- Altitude Sickness
- Alzheimer disease
- Anemia
- Angina Pectoris
- Anoxia
- Arrhythmias
- Arthritis
+ View More Disease
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