DUSP6 HEK293 Cell Transient Overexpression Lysate(Non-Denatured)
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More Files
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SDS-PAGE Gel
DUSP6 transfected lysate
Western Blot
Lane 1: DUSP6 transfected lysate ( 42 KDa).
Lane 2: Non-transfected lysate.
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Specification
Transfected Cell Line
HEK293
Plasmid
pCMV-DUSP6 full length
Host
Human
Theoretical MW (kDa)
42
Lysis Buffer
Modified RIPA Lysis Buffer:50 mM Tris-HCl pH 7.4, 150 mM NaCl, 1mM EDTA, 1% Triton X-100, 0.1% SDS, 1% Sodium deoxycholate, 1mM PMSF.
Concentration
2 mg/ml
Quality Control Testing
Transient overexpression cell lysate was tested with Anti-DUSP6 antibody (H00001848-M01) by Western Blots.
SDS-PAGE Gel
DUSP6 transfected lysate
Western Blot
Lane 1: DUSP6 transfected lysate ( 42 KDa).
Lane 2: Non-transfected lysate.Recommend Usage
Use it directly for immuno-precipitation, or heat lysate with SDS gel loading buffer to 95°C for 5 minutes followed by rapid cooling for western blot application. If dissociating conditions are required, add reducing agent prior to heating.
Storage Buffer
In modified RIPA Lysis Buffer.
Storage Instruction
Store at -80°C. Aliquot to avoid repeated freezing and thawing.
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Applications
Western Blot
Immunoprecipitation
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Gene Info — DUSP6
Entrez GeneID
1848GeneBank Accession#
BC003143Protein Accession#
AAH03143Gene Name
DUSP6
Gene Alias
MKP3, PYST1
Gene Description
dual specificity phosphatase 6
Omim ID
602748Gene Ontology
HyperlinkGene Summary
The protein encoded by this gene is a member of the dual specificity protein phosphatase subfamily. These phosphatases inactivate their target kinases by dephosphorylating both the phosphoserine/threonine and phosphotyrosine residues. They negatively regulate members of the mitogen-activated protein (MAP) kinase superfamily (MAPK/ERK, SAPK/JNK, p38), which are associated with cellular proliferation and differentiation. Different members of the family of dual specificity phosphatases show distinct substrate specificities for various MAP kinases, different tissue distribution and subcellular localization, and different modes of inducibility of their expression by extracellular stimuli. This gene product inactivates ERK2, is expressed in a variety of tissues with the highest levels in heart and pancreas, and unlike most other members of this family, is localized in the cytoplasm. Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq
Other Designations
MAP kinase phosphatase 3|serine/threonine specific protein phosphatase
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