DPF2 HEK293 Cell Transient Overexpression Lysate(Non-Denatured)
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Specification
Transfected Cell Line
HEK293
Plasmid
pCMV-DPF2 full length
Host
Human
Theoretical MW (kDa)
44
Lysis Buffer
Modified RIPA Lysis Buffer:50 mM Tris-HCl pH 7.4, 150 mM NaCl, 1mM EDTA, 1% Triton X-100, 0.1% SDS, 1% Sodium deoxycholate, 1mM PMSF.
Concentration
2 mg/ml
Quality Control Testing
Transient overexpression cell lysate was tested with Anti-DPF2 antibody (H00005977-M01) by Western Blots.
SDS-PAGE Gel
DPF2 transfected lysate
Western Blot
Lane 1: DPF2 transfected lysate ( 44 KDa).
Lane 2: Non-transfected lysate.Recommend Usage
Use it directly for immuno-precipitation, or heat lysate with SDS gel loading buffer to 95°C for 5 minutes followed by rapid cooling for western blot application. If dissociating conditions are required, add reducing agent prior to heating.
Storage Buffer
In modified RIPA Lysis Buffer.
Storage Instruction
Store at -80°C. Aliquot to avoid repeated freezing and thawing.
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Applications
Western Blot
Immunoprecipitation
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Gene Info — DPF2
Entrez GeneID
5977GeneBank Accession#
BC014889Protein Accession#
AAH14889Gene Name
DPF2
Gene Alias
MGC10180, REQ, UBID4, ubi-d4
Gene Description
D4, zinc and double PHD fingers family 2
Omim ID
601671Gene Ontology
HyperlinkGene Summary
The protein encoded by this gene is a member of the d4 domain family, characterized by a zinc finger-like structural motif. This protein functions as a transcription factor which is necessary for the apoptotic response following deprivation of survival factors. It likely serves a regulatory role in rapid hematopoietic cell growth and turnover. This gene is considered a candidate gene for multiple endocrine neoplasia type I, an inherited cancer syndrome involving multiple parathyroid, enteropancreatic, and pituitary tumors. [provided by RefSeq
Other Designations
apoptosis response zinc finger protein|requiem, apoptosis response zinc finger
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