DNMT2 HEK293 Cell Transient Overexpression Lysate(Non-Denatured)
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More Files
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Specification
Transfected Cell Line
HEK293
Plasmid
pCMV-DNMT2 full length
Host
Human
Theoretical MW (kDa)
45
Lysis Buffer
Modified RIPA Lysis Buffer:50 mM Tris-HCl pH 7.4, 150 mM NaCl, 1mM EDTA, 1% Triton X-100, 0.1% SDS, 1% Sodium deoxycholate, 1mM PMSF.
Concentration
2 mg/ml
Quality Control Testing
Transient overexpression cell lysate was tested with Anti-DNMT2 antibody (H00001787-M01) by Western Blots.
SDS-PAGE Gel
DNMT2 transfected lysate
Western Blot
Lane 1: DNMT2 transfected lysate ( 45 KDa).
Lane 2: Non-transfected lysate.Recommend Usage
Use it directly for immuno-precipitation, or heat lysate with SDS gel loading buffer to 95°C for 5 minutes followed by rapid cooling for western blot application. If dissociating conditions are required, add reducing agent prior to heating.
Storage Buffer
In modified RIPA Lysis Buffer.
Storage Instruction
Store at -80°C. Aliquot to avoid repeated freezing and thawing.
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Applications
Western Blot
Immunoprecipitation
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Gene Info — TRDMT1
Entrez GeneID
1787GeneBank Accession#
BC047733Protein Accession#
AAH47733Gene Name
TRDMT1
Gene Alias
DNMT2, M.HsaIIP, PuMet, RNMT1
Gene Description
tRNA aspartic acid methyltransferase 1
Omim ID
602478Gene Ontology
HyperlinkGene Summary
This gene encodes a protein responsible for the methylation of aspartic acid transfer RNA. While similar in sequence and structure to DNA cytosine methyltransferases, this gene is distinct and highly conserved in its function among taxa. Alternative splicing results in multiple transcript variants encoding different isoforms. [provided by RefSeq
Other Designations
DNA (cytosine-5-)-methyltransferase 2|DNA MTase homolog HsaIIP|DNA methyltransferase homolog HsaIIP|DNA methyltransferase-2|OTTHUMP00000019228|OTTHUMP00000045198|tRNA aspartic acid methyltransferase 1 variant 1|tRNA aspartic acid methyltransferase 1 varia
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Interactome
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Pathway
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Disease
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