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DNMT2 HEK293 Cell Transient Overexpression Lysate(Non-Denatured)

  • Catalog # : L075T6
  • Visit Frequency :
  • Countries :
  • Specification
  • Transfected Cell Line:
  • HEK293
  • Plasmid:
  • pCMV-DNMT2 full length
  • Host:
  • Human
  • Theoretical MW (kDa):
  • 45
  • Lysis Buffer:
  • Modified RIPA Lysis Buffer:50 mM Tris-HCl pH 7.4, 150 mM NaCl, 1mM EDTA, 1% Triton X-100, 0.1% SDS, 1% Sodium deoxycholate, 1mM PMSF.
  • Concentration:
  • 2 mg/ml
  • Quality Control Testing:
  • Transient overexpression cell lysate was tested with Anti-DNMT2 antibody (H00001787-M01) by Western Blots.
    SDS-PAGE Gel
    QC Testing of L075T6
    DNMT2 transfected lysate
    Western Blot
    QC Testing of L075T6
    Lane 1: DNMT2 transfected lysate ( 45 KDa).
    Lane 2: Non-transfected lysate.
  • Recommend Usage:
  • Use it directly for immuno-precipitation, or heat lysate with SDS gel loading buffer to 95°C for 5 minutes followed by rapid cooling for western blot application. If dissociating conditions are required, add reducing agent prior to heating.
  • Storage Buffer:
  • In modified RIPA Lysis Buffer.
  • Storage Instruction:
  • Store at -80°C. Aliquot to avoid repeated freezing and thawing.
  • Applications
  • Western Blot
  • Application Image
  • Western Blot
  • Immunoprecipitation
  • Gene Information
  • Entrez GeneID:
  • 1787
  • Gene Name:
  • TRDMT1
  • Gene Alias:
  • DNMT2,M.HsaIIP,PuMet,RNMT1
  • Gene Description:
  • tRNA aspartic acid methyltransferase 1
  • Gene Summary:
  • This gene encodes a protein responsible for the methylation of aspartic acid transfer RNA. While similar in sequence and structure to DNA cytosine methyltransferases, this gene is distinct and highly conserved in its function among taxa. Alternative splicing results in multiple transcript variants encoding different isoforms. [provided by RefSeq
  • Other Designations:
  • DNA (cytosine-5-)-methyltransferase 2,DNA MTase homolog HsaIIP,DNA methyltransferase homolog HsaIIP,DNA methyltransferase-2,OTTHUMP00000019228,OTTHUMP00000045198,tRNA aspartic acid methyltransferase 1 variant 1,tRNA aspartic acid methyltransferase 1 varia
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