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DNM1L HEK293 Cell Transient Overexpression Lysate(Non-Denatured)

  • Catalog # : L074T6
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  • Specification
  • Transfected Cell Line:
  • HEK293
  • Plasmid:
  • pCMV-DNM1L full length
  • Host:
  • Human
  • Theoretical MW (kDa):
  • 79
  • Lysis Buffer:
  • Modified RIPA Lysis Buffer:50 mM Tris-HCl pH 7.4, 150 mM NaCl, 1mM EDTA, 1% Triton X-100, 0.1% SDS, 1% Sodium deoxycholate, 1mM PMSF.
  • Concentration:
  • 2 mg/ml
  • Quality Control Testing:
  • Transient overexpression cell lysate was tested with Anti-DNM1L antibody (H00010059-M01) by Western Blots.
    SDS-PAGE Gel
    QC Testing of L074T6
    DNM1L transfected lysate
    Western Blot
    QC Testing of L074T6
    Lane 1: DNM1L transfected lysate ( 79 KDa).
    Lane 2: Non-transfected lysate.
  • Recommend Usage:
  • Use it directly for immuno-precipitation, or heat lysate with SDS gel loading buffer to 95°C for 5 minutes followed by rapid cooling for western blot application. If dissociating conditions are required, add reducing agent prior to heating.
  • Storage Buffer:
  • In modified RIPA Lysis Buffer.
  • Storage Instruction:
  • Store at -80°C. Aliquot to avoid repeated freezing and thawing.
  • Applications
  • Western Blot
  • Application Image
  • Western Blot
  • Immunoprecipitation
  • Gene Information
  • Gene Name:
  • DNM1L
  • Gene Alias:
  • DLP1,DRP1,DVLP,DYMPLE,DYNIV-11,FLJ41912,HDYNIV,VPS1
  • Gene Description:
  • dynamin 1-like
  • Gene Summary:
  • The protein encoded by this gene is a member of the dynamin superfamily of GTPases. Members of the dynamin-related subfamily, including the S. cerevisiae proteins Dnm1 and Vps1, contain the N-terminal tripartite GTPase domain but do not have the pleckstrin homology or proline-rich domains. This protein establishes mitochondrial morphology through a role in distributing mitochondrial tubules throughout the cytoplasm. The gene has 3 alternatively spliced transcripts encoding different isoforms. These transcripts are alternatively polyadenylated. [provided by RefSeq
  • Other Designations:
  • Dnm1p/Vps1p-like protein,dynamin 1-like protein,dynamin family member proline-rich carboxyl-terminal domain less,dynamin-like protein 4,dynamin-like protein IV,dynamin-related protein 1
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