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DNAJB6 HEK293 Cell Transient Overexpression Lysate(Non-Denatured)

  • Catalog # : L073T6
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  • Specification
  • Transfected Cell Line:
  • HEK293
  • Plasmid:
  • pCMV-DNAJB6 full length
  • Host:
  • Human
  • Theoretical MW (kDa):
  • 27
  • Lysis Buffer:
  • Modified RIPA Lysis Buffer:50 mM Tris-HCl pH 7.4, 150 mM NaCl, 1mM EDTA, 1% Triton X-100, 0.1% SDS, 1% Sodium deoxycholate, 1mM PMSF.
  • Concentration:
  • 2 mg/ml
  • Quality Control Testing:
  • Transient overexpression cell lysate was tested with Anti-DNAJB6 antibody (H00010049-M01) by Western Blots.
    SDS-PAGE Gel
    QC Testing of L073T6
    DNAJB6 transfected lysate
    Western Blot
    QC Testing of L073T6
    Lane 1: DNAJB6 transfected lysate ( 27 KDa).
    Lane 2: Non-transfected lysate.
  • Recommend Usage:
  • Use it directly for immuno-precipitation, or heat lysate with SDS gel loading buffer to 95°C for 5 minutes followed by rapid cooling for western blot application. If dissociating conditions are required, add reducing agent prior to heating.
  • Storage Buffer:
  • In modified RIPA Lysis Buffer.
  • Storage Instruction:
  • Store at -80°C. Aliquot to avoid repeated freezing and thawing.
  • Applications
  • Western Blot
  • Application Image
  • Western Blot
  • Immunoprecipitation
  • Gene Information
  • Gene Name:
  • DNAJB6
  • Gene Alias:
  • DJ4,DKFZp566D0824,DnaJ,FLJ42837,HHDJ1,HSJ-2,HSJ2,MGC1152,MGC117297,MRJ,MSJ-1
  • Gene Description:
  • DnaJ (Hsp40) homolog, subfamily B, member 6
  • Gene Summary:
  • This gene encodes a member of the DNAJ protein family. DNAJ family members are characterized by a highly conserved amino acid stretch called the 'J-domain' and function as one of the two major classes of molecular chaperones involved in a wide range of cellular events, such as protein folding and oligomeric protein complex assembly. This family member may also play a role in polyglutamine aggregation in specific neurons. Alternative splicing of this gene results in multiple transcript variants; however, not all variants have been fully described. [provided by RefSeq
  • Other Designations:
  • DnaJ-like 2 protein,heat shock protein J2
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