DNAI2 HEK293 Cell Transient Overexpression Lysate(Non-Denatured)
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Specification
Transfected Cell Line
HEK293
Plasmid
pCMV-DNAI2 full length
Host
Human
Theoretical MW (kDa)
67
Lysis Buffer
Modified RIPA Lysis Buffer:50 mM Tris-HCl pH 7.4, 150 mM NaCl, 1mM EDTA, 1% Triton X-100, 0.1% SDS, 1% Sodium deoxycholate, 1mM PMSF.
Concentration
2 mg/ml
Quality Control Testing
Transient overexpression cell lysate was tested with Anti-DNAI2 antibody (H00064446-M01) by Western Blots.
SDS-PAGE Gel
DNAI2 transfected lysate
Western Blot
Lane 1: DNAI2 transfected lysate ( 67 KDa).
Lane 2: Non-transfected lysate.Recommend Usage
Use it directly for immuno-precipitation, or heat lysate with SDS gel loading buffer to 95°C for 5 minutes followed by rapid cooling for western blot application. If dissociating conditions are required, add reducing agent prior to heating.
Storage Buffer
In modified RIPA Lysis Buffer.
Storage Instruction
Store at -80°C. Aliquot to avoid repeated freezing and thawing.
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Applications
Western Blot
Immunoprecipitation
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Gene Info — DNAI2
Entrez GeneID
64446GeneBank Accession#
BC039582Protein Accession#
AAH39582Gene Name
DNAI2
Gene Alias
CILD9
Gene Description
dynein, axonemal, intermediate chain 2
Omim ID
605483Gene Ontology
HyperlinkGene Summary
axonemal
Other Designations
dynein intermediate chain 2|dynein, axonemal, intermediate polypeptide 2
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Disease
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