DLX3 HEK293 Cell Transient Overexpression Lysate(Non-Denatured)
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More Files
- More Functions
SDS-PAGE Gel
DLX3 transfected lysate
Western Blot
Lane 1: DLX3 transfected lysate ( 32 KDa).
Lane 2: Non-transfected lysate.
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Specification
Transfected Cell Line
HEK293
Plasmid
pCMV-DLX3 full length
Host
Human
Theoretical MW (kDa)
32
Lysis Buffer
Modified RIPA Lysis Buffer:50 mM Tris-HCl pH 7.4, 150 mM NaCl, 1mM EDTA, 1% Triton X-100, 0.1% SDS, 1% Sodium deoxycholate, 1mM PMSF.
Concentration
2 mg/ml
Quality Control Testing
Transient overexpression cell lysate was tested with Anti-DLX3 antibody (H00001747-M01) by Western Blots.
SDS-PAGE Gel
DLX3 transfected lysate
Western Blot
Lane 1: DLX3 transfected lysate ( 32 KDa).
Lane 2: Non-transfected lysate.Recommend Usage
Use it directly for immuno-precipitation, or heat lysate with SDS gel loading buffer to 95°C for 5 minutes followed by rapid cooling for western blot application. If dissociating conditions are required, add reducing agent prior to heating.
Storage Buffer
In modified RIPA Lysis Buffer.
Storage Instruction
Store at -80°C. Aliquot to avoid repeated freezing and thawing.
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Applications
Western Blot
Immunoprecipitation
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Gene Info — DLX3
Entrez GeneID
1747GeneBank Accession#
BC012361Protein Accession#
AAH12361Gene Name
DLX3
Gene Alias
AI4, TDO
Gene Description
distal-less homeobox 3
Gene Ontology
HyperlinkGene Summary
Many vertebrate homeo box-containing genes have been identified on the basis of their sequence similarity with Drosophila developmental genes. Members of the Dlx gene family contain a homeobox that is related to that of Distal-less (Dll), a gene expressed in the head and limbs of the developing fruit fly. The Distal-less (Dlx) family of genes comprises at least 6 different members, DLX1-DLX6. Trichodentoosseous syndrome (TDO), an autosomal dominant condition, has been correlated with DLX3 gene mutation. This gene is located in a tail-to-tail configuration with another member of the gene family on the long arm of chromosome 17. Mutations in this gene have been associated with the autosomal dominant conditions trichodentoosseous syndrome and amelogenesis imperfecta with taurodontism. [provided by RefSeq
Other Designations
distal-less homeo box 3
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Interactome
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Disease
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