Transient overexpression cell lysate was tested with Anti-DDX56 antibody (H00054606-M05) by Western Blots. SDS-PAGE Gel
DDX56 transfected lysate Western Blot
Lane 1: DDX56 transfected lysate ( 62 KDa). Lane 2: Non-transfected lysate.
Use it directly for immuno-precipitation, or heat lysate with SDS gel loading buffer to 95°C for 5 minutes followed by rapid cooling for western blot application. If dissociating conditions are required, add reducing agent prior to heating.
In modified RIPA Lysis Buffer.
Store at -80°C. Aliquot to avoid repeated freezing and thawing.
This gene encodes a member of the DEAD box protein family. DEAD box proteins, characterized by the conserved motif Asp-Glu-Ala-Asp (DEAD), are putative RNA helicases. They are implicated in a number of cellular processes involving alteration of RNA secondary structure such as translation initiation, nuclear and mitochondrial splicing, and ribosome and spliceosome assembly. Based on their distribution patterns, some members of this family are believed to be involved in embryogenesis, spermatogenesis, and cellular growth and division. The protein encoded by this gene shows ATPase activity in the presence of polynucleotides and associates with nucleoplasmic 65S preribosomal particles. This gene may be involved in ribosome synthesis, most likely during assembly of the large 60S ribosomal subunit. [provided by RefSeq