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DDX41 HEK293 Cell Transient Overexpression Lysate(Non-Denatured)

  • Catalog # : L067T6
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  • Specification
  • Transfected Cell Line:
  • HEK293
  • Plasmid:
  • pCMV-DDX41 full length
  • Host:
  • Human
  • Theoretical MW (kDa):
  • 70
  • Lysis Buffer:
  • Modified RIPA Lysis Buffer:50 mM Tris-HCl pH 7.4, 150 mM NaCl, 1mM EDTA, 1% Triton X-100, 0.1% SDS, 1% Sodium deoxycholate, 1mM PMSF.
  • Concentration:
  • 2 mg/ml
  • Quality Control Testing:
  • Transient overexpression cell lysate was tested with Anti-DDX41 antibody (H00051428-M01) by Western Blots.
    SDS-PAGE Gel
    QC Testing of L067T6
    DDX41 transfected lysate
    Western Blot
    QC Testing of L067T6
    Lane 1: DDX41 transfected lysate ( 70 KDa).
    Lane 2: Non-transfected lysate.
  • Recommend Usage:
  • Use it directly for immuno-precipitation, or heat lysate with SDS gel loading buffer to 95°C for 5 minutes followed by rapid cooling for western blot application. If dissociating conditions are required, add reducing agent prior to heating.
  • Storage Buffer:
  • In modified RIPA Lysis Buffer.
  • Storage Instruction:
  • Store at -80°C. Aliquot to avoid repeated freezing and thawing.
  • Applications
  • Western Blot
  • Application Image
  • Western Blot
  • Immunoprecipitation
  • Gene Information
  • Gene Name:
  • DDX41
  • Gene Alias:
  • ABS,MGC8828
  • Gene Description:
  • DEAD (Asp-Glu-Ala-Asp) box polypeptide 41
  • Gene Summary:
  • DEAD box proteins, characterized by the conserved motif Asp-Glu-Ala-Asp (DEAD), are putative RNA helicases. They are implicated in a number of cellular processes involving alteration of RNA secondary structure, such as translation initiation, nuclear and mitochondrial splicing, and ribosome and spliceosome assembly. Based on their distribution patterns, some members of the DEAD box protein family are believed to be involved in embryogenesis, spermatogenesis, and cellular growth and division. This gene encodes a member of this family. The function of this member has not been determined. Based on studies in Drosophila, the abstrakt gene is widely required during post-transcriptional gene expression. [provided by RefSeq
  • Other Designations:
  • 2900024F02Rik,DEAD-box protein abstrakt,putative RNA helicase
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