DDT HEK293 Cell Transient Overexpression Lysate(Non-Denatured)
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Specification
Transfected Cell Line
HEK293
Plasmid
pCMV-DDT full length
Host
Human
Theoretical MW (kDa)
13
Lysis Buffer
Modified RIPA Lysis Buffer:50 mM Tris-HCl pH 7.4, 150 mM NaCl, 1mM EDTA, 1% Triton X-100, 0.1% SDS, 1% Sodium deoxycholate, 1mM PMSF.
Concentration
2 mg/ml
Quality Control Testing
Transient overexpression cell lysate was tested with Anti-DDT antibody () by Western Blots.
SDS-PAGE Gel
DDT transfected lysate
Western Blot
Lane 1: DDT transfected lysate ( 13 KDa).
Lane 2: Non-transfected lysate.Recommend Usage
Use it directly for immuno-precipitation, or heat lysate with SDS gel loading buffer to 95°C for 5 minutes followed by rapid cooling for western blot application. If dissociating conditions are required, add reducing agent prior to heating.
Storage Buffer
In modified RIPA Lysis Buffer.
Storage Instruction
Store at -80°C. Aliquot to avoid repeated freezing and thawing.
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Applications
Western Blot
Immunoprecipitation
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Gene Info — DDT
Entrez GeneID
1652GeneBank Accession#
BC005971Protein Accession#
AAH05971Gene Name
DDT
Gene Alias
DDCT
Gene Description
D-dopachrome tautomerase
Omim ID
602750Gene Ontology
HyperlinkGene Summary
D-dopachrome tautomerase converts D-dopachrome into 5,6-dihydroxyindole. The DDT gene is related to the migration inhibitory factor (MIF) in terms of sequence, enzyme activity, and gene structure. DDT and MIF are closely linked on chromosome 22. [provided by RefSeq
Other Designations
D-dopachrome decarboxylase
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Interactome
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