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COL6A2 HEK293 Cell Transient Overexpression Lysate(Non-Denatured)

  • Catalog # : L064T6
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  • Specification
  • Transfected Cell Line:
  • HEK293
  • Plasmid:
  • pCMV-COL6A2 full length
  • Host:
  • Human
  • Theoretical MW (kDa):
  • 109
  • Lysis Buffer:
  • Modified RIPA Lysis Buffer:50 mM Tris-HCl pH 7.4, 150 mM NaCl, 1mM EDTA, 1% Triton X-100, 0.1% SDS, 1% Sodium deoxycholate, 1mM PMSF.
  • Concentration:
  • 2 mg/ml
  • Quality Control Testing:
  • Transient overexpression cell lysate was tested with Anti-COL6A2 antibody (H00001292-M01) by Western Blots.
    SDS-PAGE Gel
    QC Testing of L064T6
    COL6A2 transfected lysate
    Western Blot
    QC Testing of L064T6
    Lane 1: COL6A2 transfected lysate ( 109 KDa).
    Lane 2: Non-transfected lysate.
  • Recommend Usage:
  • Use it directly for immuno-precipitation, or heat lysate with SDS gel loading buffer to 95°C for 5 minutes followed by rapid cooling for western blot application. If dissociating conditions are required, add reducing agent prior to heating.
  • Storage Buffer:
  • In modified RIPA Lysis Buffer.
  • Storage Instruction:
  • Store at -80°C. Aliquot to avoid repeated freezing and thawing.
  • Applications
  • Western Blot
  • Application Image
  • Western Blot
  • Immunoprecipitation
  • Gene Information
  • Entrez GeneID:
  • 1292
  • Gene Name:
  • COL6A2
  • Gene Alias:
  • DKFZp586E1322,FLJ46862,PP3610
  • Gene Description:
  • collagen, type VI, alpha 2
  • Gene Summary:
  • This gene encodes one of the three alpha chains of type VI collagen, a beaded filament collagen found in most connective tissues. The product of this gene contains several domains similar to von Willebrand Factor type A domains. These domains have been shown to bind extracellular matrix proteins, an interaction that explains the importance of this collagen in organizing matrix components. Mutations in this gene are associated with Bethlem myopathy and Ullrich scleroatonic muscular dystrophy. Three transcript variants have been identified for this gene. [provided by RefSeq
  • Other Designations:
  • OTTHUMP00000115569,alpha 2 type VI collagen,collagen VI, alpha-2 polypeptide,collagen alpha-2(VI) chain,human mRNA for collagen VI alpha-2 C-terminal globular domain
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