COL6A2 HEK293 Cell Transient Overexpression Lysate(Non-Denatured)
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Specification
Transfected Cell Line
HEK293
Plasmid
pCMV-COL6A2 full length
Host
Human
Theoretical MW (kDa)
109
Lysis Buffer
Modified RIPA Lysis Buffer:50 mM Tris-HCl pH 7.4, 150 mM NaCl, 1mM EDTA, 1% Triton X-100, 0.1% SDS, 1% Sodium deoxycholate, 1mM PMSF.
Concentration
2 mg/ml
Quality Control Testing
Transient overexpression cell lysate was tested with Anti-COL6A2 antibody (H00001292-M01) by Western Blots.
SDS-PAGE Gel
COL6A2 transfected lysate
Western Blot
Lane 1: COL6A2 transfected lysate ( 109 KDa).
Lane 2: Non-transfected lysate.Recommend Usage
Use it directly for immuno-precipitation, or heat lysate with SDS gel loading buffer to 95°C for 5 minutes followed by rapid cooling for western blot application. If dissociating conditions are required, add reducing agent prior to heating.
Storage Buffer
In modified RIPA Lysis Buffer.
Storage Instruction
Store at -80°C. Aliquot to avoid repeated freezing and thawing.
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Applications
Western Blot
Immunoprecipitation
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Gene Info — COL6A2
Entrez GeneID
1292GeneBank Accession#
BC002484Protein Accession#
AAH02484Gene Name
COL6A2
Gene Alias
DKFZp586E1322, FLJ46862, PP3610
Gene Description
collagen, type VI, alpha 2
Gene Ontology
HyperlinkGene Summary
This gene encodes one of the three alpha chains of type VI collagen, a beaded filament collagen found in most connective tissues. The product of this gene contains several domains similar to von Willebrand Factor type A domains. These domains have been shown to bind extracellular matrix proteins, an interaction that explains the importance of this collagen in organizing matrix components. Mutations in this gene are associated with Bethlem myopathy and Ullrich scleroatonic muscular dystrophy. Three transcript variants have been identified for this gene. [provided by RefSeq
Other Designations
OTTHUMP00000115569|alpha 2 type VI collagen|collagen VI, alpha-2 polypeptide|collagen alpha-2(VI) chain|human mRNA for collagen VI alpha-2 C-terminal globular domain
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Interactome
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