Transient overexpression cell lysate was tested with Anti-COL6A2 antibody (H00001292-M01) by Western Blots. SDS-PAGE Gel
COL6A2 transfected lysate Western Blot
Lane 1: COL6A2 transfected lysate ( 109 KDa). Lane 2: Non-transfected lysate.
Use it directly for immuno-precipitation, or heat lysate with SDS gel loading buffer to 95°C for 5 minutes followed by rapid cooling for western blot application. If dissociating conditions are required, add reducing agent prior to heating.
In modified RIPA Lysis Buffer.
Store at -80°C. Aliquot to avoid repeated freezing and thawing.
This gene encodes one of the three alpha chains of type VI collagen, a beaded filament collagen found in most connective tissues. The product of this gene contains several domains similar to von Willebrand Factor type A domains. These domains have been shown to bind extracellular matrix proteins, an interaction that explains the importance of this collagen in organizing matrix components. Mutations in this gene are associated with Bethlem myopathy and Ullrich scleroatonic muscular dystrophy. Three transcript variants have been identified for this gene. [provided by RefSeq
OTTHUMP00000115569,alpha 2 type VI collagen,collagen VI, alpha-2 polypeptide,collagen alpha-2(VI) chain,human mRNA for collagen VI alpha-2 C-terminal globular domain