CNAP1 HEK293 Cell Transient Overexpression Lysate(Non-Denatured)
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Specification
Transfected Cell Line
HEK293
Plasmid
pCMV-CNAP1 full length
Host
Human
Theoretical MW (kDa)
157
Lysis Buffer
Modified RIPA Lysis Buffer:50 mM Tris-HCl pH 7.4, 150 mM NaCl, 1mM EDTA, 1% Triton X-100, 0.1% SDS, 1% Sodium deoxycholate, 1mM PMSF.
Concentration
2 mg/ml
Quality Control Testing
Transient overexpression cell lysate was tested with Anti-CNAP1 antibody (H00009918-M01) by Western Blots.
SDS-PAGE Gel
CNAP1 transfected lysate
Western Blot
Lane 1: CNAP1 transfected lysate ( 157 KDa).
Lane 2: Non-transfected lysate.Recommend Usage
Use it directly for immuno-precipitation, or heat lysate with SDS gel loading buffer to 95°C for 5 minutes followed by rapid cooling for western blot application. If dissociating conditions are required, add reducing agent prior to heating.
Storage Buffer
In modified RIPA Lysis Buffer.
Storage Instruction
Store at -80°C. Aliquot to avoid repeated freezing and thawing.
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Applications
Western Blot
Immunoprecipitation
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Gene Info — NCAPD2
Entrez GeneID
9918GeneBank Accession#
BC028182Protein Accession#
AAH28182Gene Name
NCAPD2
Gene Alias
CAP-D2, CNAP1, KIAA0159, hCAP-D2
Gene Description
non-SMC condensin I complex, subunit D2
Gene Ontology
HyperlinkGene Summary
O
Other Designations
chromosome condensation related SMC associated protein 1|chromosome condensation-related SMC-associated protein 1
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