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Last updated: 2017/12/10

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ARRB2 HEK293 Cell Transient Overexpression Lysate(Non-Denatured)

  • Catalog # : L061T6
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  • Specification
  • Transfected Cell Line:
  • HEK293
  • Plasmid:
  • pCMV-ARRB2 full length
  • Host:
  • Human
  • Theoretical MW (kDa):
  • 46
  • Lysis Buffer:
  • Modified RIPA Lysis Buffer:50 mM Tris-HCl pH 7.4, 150 mM NaCl, 1mM EDTA, 1% Triton X-100, 0.1% SDS, 1% Sodium deoxycholate, 1mM PMSF.
  • Concentration:
  • 2 mg/ml
  • Quality Control Testing:
  • Transient overexpression cell lysate was tested with Anti-ARRB2 antibody (H00000409-M01) by Western Blots.
    SDS-PAGE Gel
    QC Testing of L061T6
    ARRB2 transfected lysate
    Western Blot
    QC Testing of L061T6
    Lane 1: ARRB2 transfected lysate ( 46 KDa).
    Lane 2: Non-transfected lysate.
  • Recommend Usage:
  • Use it directly for immuno-precipitation, or heat lysate with SDS gel loading buffer to 95°C for 5 minutes followed by rapid cooling for western blot application. If dissociating conditions are required, add reducing agent prior to heating.
  • Storage Buffer:
  • In modified RIPA Lysis Buffer.
  • Storage Instruction:
  • Store at -80°C. Aliquot to avoid repeated freezing and thawing.
  • Applications
  • Western Blot
  • Application Image
  • Western Blot
  • Immunoprecipitation
  • Gene Information
  • Entrez GeneID:
  • 409
  • Gene Name:
  • ARRB2
  • Gene Alias:
  • ARB2,ARR2,BARR2,DKFZp686L0365
  • Gene Description:
  • arrestin, beta 2
  • Gene Summary:
  • Members of arrestin/beta-arrestin protein family are thought to participate in agonist-mediated desensitization of G-protein-coupled receptors and cause specific dampening of cellular responses to stimuli such as hormones, neurotransmitters, or sensory signals. Arrestin beta 2, like arrestin beta 1, was shown to inhibit beta-adrenergic receptor function in vitro. It is expressed at high levels in the central nervous system and may play a role in the regulation of synaptic receptors. Besides the brain, a cDNA for arrestin beta 2 was isolated from thyroid gland, and thus it may also be involved in hormone-specific desensitization of TSH receptors. Multiple alternatively spliced transcript variants have been found for this gene, but the full-length nature of some variants has not been defined. [provided by RefSeq
  • Other Designations:
  • arrestin 3
  • Interactome
  • Interactome
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