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CRX HEK293 Cell Transient Overexpression Lysate(Non-Denatured)

  • Catalog # : L060T6
  • Visit Frequency :
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  • Specification
  • Transfected Cell Line:
  • HEK293
  • Plasmid:
  • pCMV-CRX full length
  • Host:
  • Human
  • Theoretical MW (kDa):
  • 32
  • Lysis Buffer:
  • Modified RIPA Lysis Buffer:50 mM Tris-HCl pH 7.4, 150 mM NaCl, 1mM EDTA, 1% Triton X-100, 0.1% SDS, 1% Sodium deoxycholate, 1mM PMSF.
  • Concentration:
  • 2 mg/ml
  • Quality Control Testing:
  • Transient overexpression cell lysate was tested with Anti-CRX antibody (H00001406-M02) by Western Blots.
    SDS-PAGE Gel
    QC Testing of L060T6
    CRX transfected lysate
    Western Blot
    QC Testing of L060T6
    Lane 1: CRX transfected lysate ( 32 KDa).
    Lane 2: Non-transfected lysate.
  • Recommend Usage:
  • Use it directly for immuno-precipitation, or heat lysate with SDS gel loading buffer to 95°C for 5 minutes followed by rapid cooling for western blot application. If dissociating conditions are required, add reducing agent prior to heating.
  • Storage Buffer:
  • In modified RIPA Lysis Buffer.
  • Storage Instruction:
  • Store at -80°C. Aliquot to avoid repeated freezing and thawing.
  • Applications
  • Western Blot
  • Application Image
  • Western Blot
  • Immunoprecipitation
  • Gene Information
  • Entrez GeneID:
  • 1406
  • Gene Name:
  • CRX
  • Gene Alias:
  • CORD2,CRD,LCA7,OTX3
  • Gene Description:
  • cone-rod homeobox
  • Gene Summary:
  • The protein encoded by this gene is a photoreceptor-specific transcription factor which plays a role in the differentiation of photoreceptor cells. This homeodomain protein is necessary for the maintenance of normal cone and rod function. Mutations in this gene are associated with photoreceptor degeneration, Leber congenital amaurosis type III and the autosomal dominant cone-rod dystrophy 2. Several alternatively spliced transcript variants of this gene have been described, but the full-length nature of some variants has not been determined. [provided by RefSeq
  • Other Designations:
  • cone-rod homeobox protein,orthodenticle homeobox 3
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