FES HEK293 Cell Transient Overexpression Lysate(Non-Denatured)
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More Files
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Specification
Transfected Cell Line
HEK293
Plasmid
pCMV-FES full length
Host
Human
Theoretical MW (kDa)
93
Lysis Buffer
Modified RIPA Lysis Buffer:50 mM Tris-HCl pH 7.4, 150 mM NaCl, 1mM EDTA, 1% Triton X-100, 0.1% SDS, 1% Sodium deoxycholate, 1mM PMSF.
Concentration
2 mg/ml
Quality Control Testing
Transient overexpression cell lysate was tested with Anti-FES antibody (H00002242-M01) by Western Blots.
SDS-PAGE Gel
FES transfected lysate
Western Blot
Lane 1: FES transfected lysate ( 93 KDa).
Lane 2: Non-transfected lysate.Recommend Usage
Use it directly for immuno-precipitation, or heat lysate with SDS gel loading buffer to 95°C for 5 minutes followed by rapid cooling for western blot application. If dissociating conditions are required, add reducing agent prior to heating.
Storage Buffer
In modified RIPA Lysis Buffer.
Storage Instruction
Store at -80°C. Aliquot to avoid repeated freezing and thawing.
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Applications
Western Blot
Immunoprecipitation
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Gene Info — FES
Entrez GeneID
2242GeneBank Accession#
BC035357Protein Accession#
AAH35357Gene Name
FES
Gene Alias
FPS
Gene Description
feline sarcoma oncogene
Omim ID
190030Gene Ontology
HyperlinkGene Summary
This gene encodes the human cellular counterpart of a feline sarcoma retrovirus protein with transforming capabilities. The gene product has tyrosine-specific protein kinase activity and that activity is required for maintenance of cellular transformation. Its chromosomal location has linked it to a specific translocation event identified in patients with acute promyelocytic leukemia but it is also involved in normal hematopoiesis as well as growth factor and cytokine receptor signaling. Alternative splicing results in multiple variants encoding different isoforms
Other Designations
Oncogene FES, feline sarcoma virus|c-fes/fps protein|feline sarcoma (Snyder-Theilen) viral (v-fes)/Fujinami avian sarcoma (PRCII) viral (v-fps) oncogene homolog|proto-oncogene tyrosine-protein kinase Fes/Fps
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Interactome
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Pathway
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Disease
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