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CYP2J2 HEK293 Cell Transient Overexpression Lysate(Non-Denatured)

  • Catalog # : L052T6
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  • Specification
  • Transfected Cell Line:
  • HEK293
  • Plasmid:
  • pCMV-CYP2J2 full length
  • Host:
  • Human
  • Theoretical MW (kDa):
  • 58
  • Lysis Buffer:
  • Modified RIPA Lysis Buffer:50 mM Tris-HCl pH 7.4, 150 mM NaCl, 1mM EDTA, 1% Triton X-100, 0.1% SDS, 1% Sodium deoxycholate, 1mM PMSF.
  • Concentration:
  • 2 mg/ml
  • Quality Control Testing:
  • Transient overexpression cell lysate was tested with Anti-CYP2J2 antibody (H00001573-M01) by Western Blots.
    SDS-PAGE Gel
    QC Testing of L052T6
    CYP2J2 transfected lysate
    Western Blot
    QC Testing of L052T6
    Lane 1: CYP2J2 transfected lysate ( 58 KDa).
    Lane 2: Non-transfected lysate.
  • Recommend Usage:
  • Use it directly for immuno-precipitation, or heat lysate with SDS gel loading buffer to 95°C for 5 minutes followed by rapid cooling for western blot application. If dissociating conditions are required, add reducing agent prior to heating.
  • Storage Buffer:
  • In modified RIPA Lysis Buffer.
  • Storage Instruction:
  • Store at -80°C. Aliquot to avoid repeated freezing and thawing.
  • Applications
  • Western Blot
  • Application Image
  • Western Blot
  • Immunoprecipitation
  • Gene Information
  • Entrez GeneID:
  • 1573
  • Gene Name:
  • CYP2J2
  • Gene Alias:
  • CPJ2
  • Gene Description:
  • cytochrome P450, family 2, subfamily J, polypeptide 2
  • Gene Summary:
  • This gene encodes a member of the cytochrome P450 superfamily of enzymes. The cytochrome P450 proteins are monooxygenases which catalyze many reactions involved in drug metabolism and synthesis of cholesterol, steroids and other lipids. This protein localizes to the endoplasmic reticulum and is thought to be the predominant enzyme responsible for epoxidation of endogenous arachidonic acid in cardiac tissue. [provided by RefSeq
  • Other Designations:
  • OTTHUMP00000010550,arachidonic acid epoxygenase,cytochrome P450, subfamily IIJ (arachidonic acid epoxygenase) polypeptide 2,flavoprotein-linked monooxygenase,microsomal monooxygenase
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