CYGB HEK293 Cell Transient Overexpression Lysate(Non-Denatured)
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Specification
Transfected Cell Line
HEK293
Plasmid
pCMV-CYGB full length
Host
Human
Theoretical MW (kDa)
21
Lysis Buffer
Modified RIPA Lysis Buffer:50 mM Tris-HCl pH 7.4, 150 mM NaCl, 1mM EDTA, 1% Triton X-100, 0.1% SDS, 1% Sodium deoxycholate, 1mM PMSF.
Concentration
2 mg/ml
Quality Control Testing
Transient overexpression cell lysate was tested with Anti-CYGB antibody (H00114757-M02) by Western Blots.
SDS-PAGE Gel
CYGB transfected lysate
Western Blot
Lane 1: CYGB transfected lysate ( 21 KDa).
Lane 2: Non-transfected lysate.Recommend Usage
Use it directly for immuno-precipitation, or heat lysate with SDS gel loading buffer to 95°C for 5 minutes followed by rapid cooling for western blot application. If dissociating conditions are required, add reducing agent prior to heating.
Storage Buffer
In modified RIPA Lysis Buffer.
Storage Instruction
Store at -80°C. Aliquot to avoid repeated freezing and thawing.
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Applications
Western Blot
Immunoprecipitation
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Gene Info — CYGB
Entrez GeneID
114757GeneBank Accession#
BC029798Protein Accession#
AAH29798Gene Name
CYGB
Gene Alias
HGB, STAP
Gene Description
cytoglobin
Omim ID
608759Gene Ontology
HyperlinkGene Summary
Cytoglobin is a ubiquitously expressed hexacoordinate hemoglobin that may facilitate diffusion of oxygen through tissues, scavenge nitric oxide or other reactive oxygen species, or serve a protective function during oxidative stress (Trent and Hargrove, 2002 [PubMed 11893755]).[supplied by OMIM
Other Designations
histoglobin|stellate cell activation-associated protein
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