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Last updated: 2017/8/13

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DAAM1 HEK293 Cell Transient Overexpression Lysate(Non-Denatured)

  • Catalog # : L050T6
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  • Specification
  • Transfected Cell Line:
  • HEK293
  • Plasmid:
  • pCMV-DAAM1 full length
  • Host:
  • Human
  • Theoretical MW (kDa):
  • 122
  • Lysis Buffer:
  • Modified RIPA Lysis Buffer:50 mM Tris-HCl pH 7.4, 150 mM NaCl, 1mM EDTA, 1% Triton X-100, 0.1% SDS, 1% Sodium deoxycholate, 1mM PMSF.
  • Concentration:
  • 2 mg/ml
  • Quality Control Testing:
  • Transient overexpression cell lysate was tested with Anti-DAAM1 antibody (H00023002-M05) by Western Blots.
    SDS-PAGE Gel
    QC Testing of L050T6
    DAAM1 transfected lysate
    Western Blot
    QC Testing of L050T6
    Lane 1: DAAM1 transfected lysate ( 122 KDa).
    Lane 2: Non-transfected lysate.
  • Recommend Usage:
  • Use it directly for immuno-precipitation, or heat lysate with SDS gel loading buffer to 95°C for 5 minutes followed by rapid cooling for western blot application. If dissociating conditions are required, add reducing agent prior to heating.
  • Storage Buffer:
  • In modified RIPA Lysis Buffer.
  • Storage Instruction:
  • Store at -80°C. Aliquot to avoid repeated freezing and thawing.
  • Applications
  • Western Blot
  • Application Image
  • Western Blot
  • Immunoprecipitation
  • Gene Information
  • Gene Name:
  • DAAM1
  • Gene Alias:
  • FLJ41657,KIAA0666
  • Gene Description:
  • dishevelled associated activator of morphogenesis 1
  • Gene Summary:
  • Functions of the cell cortex, including motility, adhesion, and cytokinesis, are mediated by the reorganization of the actin cytoskeleton and recent evidence suggests a role for the Formin homology (FH) proteins in these processes. The protein encoded by this gene contains FH domains and belongs to a novel FH protein subfamily implicated in cell polarity. Wnt/Fz signaling activates the small GTPase Rho, a key regulator of cytoskeleton architecture, to control cell polarity and movement during development. Activation requires Dvl-Rho complex formation, an assembly mediated by this gene product, which is thought to function as a scaffolding protein. Evidence of alternative splicing has been observed for this gene but the full-length nature of these variants has not been determined. [provided by RefSeq
  • Other Designations:
  • OTTHUMP00000179033,dishevelled-associated activator of morphogenesis 1
  • Gene Pathway
  • Related Disease
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