Transient overexpression cell lysate was tested with Anti-CRADD antibody (H00008738-M01) by Western Blots. SDS-PAGE Gel
CRADD transfected lysate Western Blot
Lane 1: CRADD transfected lysate ( 23 KDa). Lane 2: Non-transfected lysate.
Use it directly for immuno-precipitation, or heat lysate with SDS gel loading buffer to 95°C for 5 minutes followed by rapid cooling for western blot application. If dissociating conditions are required, add reducing agent prior to heating.
In modified RIPA Lysis Buffer.
Store at -80°C. Aliquot to avoid repeated freezing and thawing.
The protein encoded by this gene is a death domain (CARD/DD)-containing protein and has been shown to induce cell apoptosis. Through its CARD domain, this protein interacts with, and thus recruits, caspase 2/ICH1 to the cell death signal transduction complex that includes tumor necrosis factor receptor 1 (TNFR1A), RIPK1/RIP kinase, and numbers of other CARD domain-containing proteins. [provided by RefSeq
RIP-associated ICH1/CED3-homologous protein with death domain,RIP-associated protein with a death domain,caspase and RIP adaptor with death domain,death adaptor molecule RAIDD,death domain containing protein CRADD