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Last updated: 2017/7/23

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CRADD HEK293 Cell Transient Overexpression Lysate(Non-Denatured)

  • Catalog # : L047T6
  • Visit Frequency :
  • Countries :
  • Specification
  • Transfected Cell Line:
  • HEK293
  • Plasmid:
  • pCMV-CRADD full length
  • Host:
  • Human
  • Theoretical MW (kDa):
  • 23
  • Lysis Buffer:
  • Modified RIPA Lysis Buffer:50 mM Tris-HCl pH 7.4, 150 mM NaCl, 1mM EDTA, 1% Triton X-100, 0.1% SDS, 1% Sodium deoxycholate, 1mM PMSF.
  • Concentration:
  • 2 mg/ml
  • Quality Control Testing:
  • Transient overexpression cell lysate was tested with Anti-CRADD antibody (H00008738-M01) by Western Blots.
    SDS-PAGE Gel
    QC Testing of L047T6
    CRADD transfected lysate
    Western Blot
    QC Testing of L047T6
    Lane 1: CRADD transfected lysate ( 23 KDa).
    Lane 2: Non-transfected lysate.
  • Recommend Usage:
  • Use it directly for immuno-precipitation, or heat lysate with SDS gel loading buffer to 95°C for 5 minutes followed by rapid cooling for western blot application. If dissociating conditions are required, add reducing agent prior to heating.
  • Storage Buffer:
  • In modified RIPA Lysis Buffer.
  • Storage Instruction:
  • Store at -80°C. Aliquot to avoid repeated freezing and thawing.
  • Applications
  • Western Blot
  • Application Image
  • Western Blot
  • Immunoprecipitation
  • Gene Information
  • Entrez GeneID:
  • 8738
  • Gene Name:
  • CRADD
  • Gene Alias:
  • MGC9163,RAIDD
  • Gene Description:
  • CASP2 and RIPK1 domain containing adaptor with death domain
  • Gene Summary:
  • The protein encoded by this gene is a death domain (CARD/DD)-containing protein and has been shown to induce cell apoptosis. Through its CARD domain, this protein interacts with, and thus recruits, caspase 2/ICH1 to the cell death signal transduction complex that includes tumor necrosis factor receptor 1 (TNFR1A), RIPK1/RIP kinase, and numbers of other CARD domain-containing proteins. [provided by RefSeq
  • Other Designations:
  • RIP-associated ICH1/CED3-homologous protein with death domain,RIP-associated protein with a death domain,caspase and RIP adaptor with death domain,death adaptor molecule RAIDD,death domain containing protein CRADD
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