CNOT3 HEK293 Cell Transient Overexpression Lysate(Non-Denatured)
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More Files
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Specification
Transfected Cell Line
HEK293
Plasmid
pCMV-CNOT3 full length
Host
Human
Theoretical MW (kDa)
82
Lysis Buffer
Modified RIPA Lysis Buffer:50 mM Tris-HCl pH 7.4, 150 mM NaCl, 1mM EDTA, 1% Triton X-100, 0.1% SDS, 1% Sodium deoxycholate, 1mM PMSF.
Concentration
2 mg/ml
Quality Control Testing
Transient overexpression cell lysate was tested with Anti-CNOT3 antibody (H00004849-M01) by Western Blots.
SDS-PAGE Gel
CNOT3 transfected lysate
Western Blot
Lane 1: CNOT3 transfected lysate ( 82 KDa).
Lane 2: Non-transfected lysate.Recommend Usage
Use it directly for immuno-precipitation, or heat lysate with SDS gel loading buffer to 95°C for 5 minutes followed by rapid cooling for western blot application. If dissociating conditions are required, add reducing agent prior to heating.
Storage Buffer
In modified RIPA Lysis Buffer.
Storage Instruction
Store at -80°C. Aliquot to avoid repeated freezing and thawing.
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Applications
Western Blot
Immunoprecipitation
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Gene Info — CNOT3
Entrez GeneID
4849GeneBank Accession#
NM_014516Protein Accession#
NP_055331Gene Name
CNOT3
Gene Alias
KIAA0691, LENG2, NOT3, NOT3H
Gene Description
CCR4-NOT transcription complex, subunit 3
Omim ID
604910Gene Ontology
HyperlinkGene Summary
O
Other Designations
NOT3 (negative regulator of transcription 3, yeast) homolog
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