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CBS HEK293 Cell Transient Overexpression Lysate(Non-Denatured)

  • Catalog # : L043T6
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  • Specification
  • Transfected Cell Line:
  • HEK293
  • Plasmid:
  • pCMV-CBS full length
  • Host:
  • Human
  • Theoretical MW (kDa):
  • 61
  • Lysis Buffer:
  • Modified RIPA Lysis Buffer:50 mM Tris-HCl pH 7.4, 150 mM NaCl, 1mM EDTA, 1% Triton X-100, 0.1% SDS, 1% Sodium deoxycholate, 1mM PMSF.
  • Concentration:
  • 2 mg/ml
  • Quality Control Testing:
  • Transient overexpression cell lysate was tested with Anti-CBS antibody (H00000875-M01) by Western Blots.
    SDS-PAGE Gel
    QC Testing of L043T6
    CBS transfected lysate
    Western Blot
    QC Testing of L043T6
    Lane 1: CBS transfected lysate ( 61 KDa).
    Lane 2: Non-transfected lysate.
  • Recommend Usage:
  • Use it directly for immuno-precipitation, or heat lysate with SDS gel loading buffer to 95°C for 5 minutes followed by rapid cooling for western blot application. If dissociating conditions are required, add reducing agent prior to heating.
  • Storage Buffer:
  • In modified RIPA Lysis Buffer.
  • Storage Instruction:
  • Store at -80°C. Aliquot to avoid repeated freezing and thawing.
  • Applications
  • Western Blot
  • Application Image
  • Western Blot
  • Immunoprecipitation
  • Gene Information
  • Entrez GeneID:
  • 875
  • Gene Name:
  • CBS
  • Gene Alias:
  • HIP4
  • Gene Description:
  • cystathionine-beta-synthase
  • Gene Summary:
  • The protein encoded by this gene acts as a homotetramer to catalyze the conversion of homocysteine to cystathionine, the first step in the transsulfuration pathway. The encoded protein is allosterically activated by adenosyl-methionine and uses pyridoxal phosphate as a cofactor. Defects in this gene can cause cystathionine beta-synthase deficiency (CBSD), which can lead to homocystinuria. [provided by RefSeq
  • Other Designations:
  • OTTHUMP00000109416,OTTHUMP00000109418,beta-thionase,cystathionine beta-synthase,methylcysteine synthase,serine sulfhydrase
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