BAIAP2 HEK293 Cell Transient Overexpression Lysate(Non-Denatured)
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More Files
- More Functions
SDS-PAGE Gel
BAIAP2 transfected lysate
Western Blot
Lane 1: BAIAP2 transfected lysate ( 57 KDa).
Lane 2: Non-transfected lysate.
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Specification
Transfected Cell Line
HEK293
Plasmid
pCMV-BAIAP2 full length
Host
Human
Theoretical MW (kDa)
57
Lysis Buffer
Modified RIPA Lysis Buffer:50 mM Tris-HCl pH 7.4, 150 mM NaCl, 1mM EDTA, 1% Triton X-100, 0.1% SDS, 1% Sodium deoxycholate, 1mM PMSF.
Concentration
2 mg/ml
Quality Control Testing
Transient overexpression cell lysate was tested with Anti-BAIAP2 antibody (H00010458-M01) by Western Blots.
SDS-PAGE Gel
BAIAP2 transfected lysate
Western Blot
Lane 1: BAIAP2 transfected lysate ( 57 KDa).
Lane 2: Non-transfected lysate.Recommend Usage
Use it directly for immuno-precipitation, or heat lysate with SDS gel loading buffer to 95°C for 5 minutes followed by rapid cooling for western blot application. If dissociating conditions are required, add reducing agent prior to heating.
Storage Buffer
In modified RIPA Lysis Buffer.
Storage Instruction
Store at -80°C. Aliquot to avoid repeated freezing and thawing.
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Applications
Western Blot
Immunoprecipitation
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Gene Info — BAIAP2
Entrez GeneID
10458GeneBank Accession#
BC032559Protein Accession#
AAH32559Gene Name
BAIAP2
Gene Alias
BAP2, IRSP53
Gene Description
BAI1-associated protein 2
Omim ID
605475Gene Ontology
HyperlinkGene Summary
The protein encoded by this gene has been identified as a brain-specific angiogenesis inhibitor (BAI1)-binding protein. This adaptor protein links membrane bound G-proteins to cytoplasmic effector proteins. This protein functions as an insulin receptor tyrosine kinase substrate and suggests a role for insulin in the central nervous system. It also associates with a downstream effector of Rho small G proteins, which is associated with the formation of stress fibers and cytokinesis. This protein is involved in lamellipodia and filopodia formation in motile cells and may affect neuronal growth-cone guidance. This protein has also been identified as interacting with the dentatorubral-pallidoluysian atrophy gene, which is associated with an autosomal dominant neurodegenerative disease. Alternative splicing results in multiple transcript variants encoding distinct isoforms
Other Designations
insulin receptor substrate p53
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Interactome
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Pathway
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Disease
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