Products

Transfected Cell Line

HEK293

Plasmid

pCMV-BUB1 full length

Host

Human

Theoretical MW (kDa)

122

Lysis Buffer

Modified RIPA Lysis Buffer:50 mM Tris-HCl pH 7.4, 150 mM NaCl, 1mM EDTA, 1% Triton X-100, 0.1% SDS, 1% Sodium deoxycholate, 1mM PMSF.

Concentration

2 mg/ml

Quality Control Testing

Transient overexpression cell lysate was tested with Anti-BUB1 antibody (H00000699-M02) by Western Blots.

SDS-PAGE Gel

BUB1 transfected lysate

Western Blot

Lane 1: BUB1 transfected lysate ( 122 KDa).
Lane 2: Non-transfected lysate.

Recommend Usage

Use it directly for immuno-precipitation, or heat lysate with SDS gel loading buffer to 95°C for 5 minutes followed by rapid cooling for western blot application. If dissociating conditions are required, add reducing agent prior to heating.

Storage Buffer

In modified RIPA Lysis Buffer.

Storage Instruction

Store at -80°C. Aliquot to avoid repeated freezing and thawing.