Transient overexpression cell lysate was tested with Anti-BUB1 antibody (H00000699-M02) by Western Blots. SDS-PAGE Gel
BUB1 transfected lysate Western Blot
Lane 1: BUB1 transfected lysate ( 122 KDa). Lane 2: Non-transfected lysate.
Use it directly for immuno-precipitation, or heat lysate with SDS gel loading buffer to 95°C for 5 minutes followed by rapid cooling for western blot application. If dissociating conditions are required, add reducing agent prior to heating.
In modified RIPA Lysis Buffer.
Store at -80°C. Aliquot to avoid repeated freezing and thawing.
This gene encodes a kinase involved in spindle checkpoint function. The kinase functions in part by phosphorylating a member of the miotic checkpoint complex and activating the spindle checkpoint. Mutations in this gene have been associated with aneuploidy and several forms of cancer. [provided by RefSeq
BUB1 budding uninhibited by benzimidazoles 1 homolog,budding uninhibited by benzimidazoles 1,mitotic spindle checkpoint kinase,putative serine/threonine-protein kinase