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BUB1 HEK293 Cell Transient Overexpression Lysate(Non-Denatured)

  • Catalog # : L039T6
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  • Specification
  • Transfected Cell Line:
  • HEK293
  • Plasmid:
  • pCMV-BUB1 full length
  • Host:
  • Human
  • Theoretical MW (kDa):
  • 122
  • Lysis Buffer:
  • Modified RIPA Lysis Buffer:50 mM Tris-HCl pH 7.4, 150 mM NaCl, 1mM EDTA, 1% Triton X-100, 0.1% SDS, 1% Sodium deoxycholate, 1mM PMSF.
  • Concentration:
  • 2 mg/ml
  • Quality Control Testing:
  • Transient overexpression cell lysate was tested with Anti-BUB1 antibody (H00000699-M02) by Western Blots.
    SDS-PAGE Gel
    QC Testing of L039T6
    BUB1 transfected lysate
    Western Blot
    QC Testing of L039T6
    Lane 1: BUB1 transfected lysate ( 122 KDa).
    Lane 2: Non-transfected lysate.
  • Recommend Usage:
  • Use it directly for immuno-precipitation, or heat lysate with SDS gel loading buffer to 95°C for 5 minutes followed by rapid cooling for western blot application. If dissociating conditions are required, add reducing agent prior to heating.
  • Storage Buffer:
  • In modified RIPA Lysis Buffer.
  • Storage Instruction:
  • Store at -80°C. Aliquot to avoid repeated freezing and thawing.
  • Applications
  • Western Blot
  • Application Image
  • Western Blot
  • Immunoprecipitation
  • Gene Information
  • Entrez GeneID:
  • 699
  • Gene Name:
  • BUB1
  • Gene Alias:
  • BUB1A,BUB1L,hBUB1
  • Gene Description:
  • budding uninhibited by benzimidazoles 1 homolog (yeast)
  • Gene Summary:
  • This gene encodes a kinase involved in spindle checkpoint function. The kinase functions in part by phosphorylating a member of the miotic checkpoint complex and activating the spindle checkpoint. Mutations in this gene have been associated with aneuploidy and several forms of cancer. [provided by RefSeq
  • Other Designations:
  • BUB1 budding uninhibited by benzimidazoles 1 homolog,budding uninhibited by benzimidazoles 1,mitotic spindle checkpoint kinase,putative serine/threonine-protein kinase
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  • Interactome
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