CKLFSF5 HEK293 Cell Transient Overexpression Lysate(Non-Denatured)
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Specification
Transfected Cell Line
HEK293
Plasmid
pCMV-CKLFSF5 full length
Host
Human
Theoretical MW (kDa)
17
Lysis Buffer
Modified RIPA Lysis Buffer:50 mM Tris-HCl pH 7.4, 150 mM NaCl, 1mM EDTA, 1% Triton X-100, 0.1% SDS, 1% Sodium deoxycholate, 1mM PMSF.
Concentration
2 mg/ml
Quality Control Testing
Transient overexpression cell lysate was tested with Anti-CKLFSF5 antibody (H00116173-M01) by Western Blots.
SDS-PAGE Gel
CMTM5 transfected lysate
Western Blot
Lane 1: CMTM5 transfected lysate ( 17 KDa).
Lane 2: Non-transfected lysate.Recommend Usage
Use it directly for immuno-precipitation, or heat lysate with SDS gel loading buffer to 95°C for 5 minutes followed by rapid cooling for western blot application. If dissociating conditions are required, add reducing agent prior to heating.
Storage Buffer
In modified RIPA Lysis Buffer.
Storage Instruction
Store at -80°C. Aliquot to avoid repeated freezing and thawing.
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Applications
Western Blot
Immunoprecipitation
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Gene Info — CMTM5
Entrez GeneID
116173GeneBank Accession#
BC013109Protein Accession#
AAH13109Gene Name
CMTM5
Gene Alias
CKLFSF5, FLJ37521
Gene Description
CKLF-like MARVEL transmembrane domain containing 5
Omim ID
607888Gene Ontology
HyperlinkGene Summary
This gene belongs to the chemokine-like factor gene superfamily. This family of genes encodes multi-pass membrane proteins that are similar to both the chemokine and the transmembrane 4 superfamilies of signaling molecules. Alternate transcriptional splice variants of this gene, encoding different isoforms, have been characterized. [provided by RefSeq
Other Designations
chemokine-like factor super family 5|chemokine-like factor superfamily 5
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