CHTF18 HEK293 Cell Transient Overexpression Lysate(Non-Denatured)
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Specification
Transfected Cell Line
HEK293
Plasmid
pCMV-CHTF18 full length
Host
Human
Theoretical MW (kDa)
107
Lysis Buffer
Modified RIPA Lysis Buffer:50 mM Tris-HCl pH 7.4, 150 mM NaCl, 1mM EDTA, 1% Triton X-100, 0.1% SDS, 1% Sodium deoxycholate, 1mM PMSF.
Concentration
2 mg/ml
Quality Control Testing
Transient overexpression cell lysate was tested with Anti-CHTF18 antibody (H00063922-M01) by Western Blots.
SDS-PAGE Gel
CHTF18 transfected lysate
Western Blot
Lane 1: CHTF18 transfected lysate ( 107 KDa).
Lane 2: Non-transfected lysate.Recommend Usage
Use it directly for immuno-precipitation, or heat lysate with SDS gel loading buffer to 95°C for 5 minutes followed by rapid cooling for western blot application. If dissociating conditions are required, add reducing agent prior to heating.
Storage Buffer
In modified RIPA Lysis Buffer.
Storage Instruction
Store at -80°C. Aliquot to avoid repeated freezing and thawing.
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Applications
Western Blot
Immunoprecipitation
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Gene Info — CHTF18
Entrez GeneID
63922GeneBank Accession#
BC018184Protein Accession#
AAH18184Gene Name
CHTF18
Gene Alias
C16orf41, C321D2.2, C321D2.3, C321D2.4, CHL12, Ctf18, RUVBL
Gene Description
CTF18, chromosome transmission fidelity factor 18 homolog (S. cerevisiae)
Gene Ontology
HyperlinkGene Summary
O
Other Designations
C321D2.3 (novel protein)|C321D2.4 (novel protein)|CTF18, chromosome transmission fidelity factor 18 homolog|homolog of yeast CHL12|some homology with holliday junction DNA helicase RUVB like
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