CHD4 HEK293 Cell Transient Overexpression Lysate(Non-Denatured)
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Specification
Transfected Cell Line
HEK293
Plasmid
pCMV-CHD4 full length
Host
Human
Theoretical MW (kDa)
220
Lysis Buffer
Modified RIPA Lysis Buffer:50 mM Tris-HCl pH 7.4, 150 mM NaCl, 1mM EDTA, 1% Triton X-100, 0.1% SDS, 1% Sodium deoxycholate, 1mM PMSF.
Concentration
2 mg/ml
Quality Control Testing
Transient overexpression cell lysate was tested with Anti-CHD4 antibody (H00001108-M01) by Western Blots.
SDS-PAGE Gel
CHD4 transfected lysate
Western Blot
Lane 1: CHD4 transfected lysate ( 220 KDa).
Lane 2: Non-transfected lysate.Recommend Usage
Use it directly for immuno-precipitation, or heat lysate with SDS gel loading buffer to 95°C for 5 minutes followed by rapid cooling for western blot application. If dissociating conditions are required, add reducing agent prior to heating.
Storage Buffer
In modified RIPA Lysis Buffer.
Storage Instruction
Store at -80°C. Aliquot to avoid repeated freezing and thawing.
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Applications
Western Blot
Immunoprecipitation
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Gene Info — CHD4
Entrez GeneID
1108GeneBank Accession#
NM_001273Protein Accession#
NP_001264Gene Name
CHD4
Gene Alias
DKFZp686E06161, Mi-2b, Mi2-BETA
Gene Description
chromodomain helicase DNA binding protein 4
Omim ID
603277Gene Ontology
HyperlinkGene Summary
The product of this gene belongs to the SNF2/RAD54 helicase family. It represents the main component of the nucleosome remodeling and deacetylase complex and plays an important role in epigenetic transcriptional repression. Patients with dermatomyositis develop antibodies against this protein. [provided by RefSeq
Other Designations
Mi-2 autoantigen 218 kDa protein
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