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CHD4 HEK293 Cell Transient Overexpression Lysate(Non-Denatured)

  • Catalog # : L034T6
  • Visit Frequency :
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  • Specification
  • Transfected Cell Line:
  • HEK293
  • Plasmid:
  • pCMV-CHD4 full length
  • Host:
  • Human
  • Theoretical MW (kDa):
  • 220
  • Lysis Buffer:
  • Modified RIPA Lysis Buffer:50 mM Tris-HCl pH 7.4, 150 mM NaCl, 1mM EDTA, 1% Triton X-100, 0.1% SDS, 1% Sodium deoxycholate, 1mM PMSF.
  • Concentration:
  • 2 mg/ml
  • Quality Control Testing:
  • Transient overexpression cell lysate was tested with Anti-CHD4 antibody (H00001108-M01) by Western Blots.
    SDS-PAGE Gel
    QC Testing of L034T6
    CHD4 transfected lysate
    Western Blot
    QC Testing of L034T6
    Lane 1: CHD4 transfected lysate ( 220 KDa).
    Lane 2: Non-transfected lysate.
  • Recommend Usage:
  • Use it directly for immuno-precipitation, or heat lysate with SDS gel loading buffer to 95°C for 5 minutes followed by rapid cooling for western blot application. If dissociating conditions are required, add reducing agent prior to heating.
  • Storage Buffer:
  • In modified RIPA Lysis Buffer.
  • Storage Instruction:
  • Store at -80°C. Aliquot to avoid repeated freezing and thawing.
  • Applications
  • Western Blot
  • Application Image
  • Western Blot
  • Immunoprecipitation
  • Gene Information
  • Entrez GeneID:
  • 1108
  • Gene Name:
  • CHD4
  • Gene Alias:
  • DKFZp686E06161,Mi-2b,Mi2-BETA
  • Gene Description:
  • chromodomain helicase DNA binding protein 4
  • Gene Summary:
  • The product of this gene belongs to the SNF2/RAD54 helicase family. It represents the main component of the nucleosome remodeling and deacetylase complex and plays an important role in epigenetic transcriptional repression. Patients with dermatomyositis develop antibodies against this protein. [provided by RefSeq
  • Other Designations:
  • Mi-2 autoantigen 218 kDa protein
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