CDC2L5 HEK293 Cell Transient Overexpression Lysate(Non-Denatured)
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More Files
- More Functions
SDS-PAGE Gel
CDC2L5 transfected lysate
Western Blot
Lane 1: CDC2L5 transfected lysate ( 37 KDa).
Lane 2: Non-transfected lysate.
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Specification
Transfected Cell Line
HEK293
Plasmid
pCMV-CDC2L5 full length
Host
Human
Theoretical MW (kDa)
37
Lysis Buffer
Modified RIPA Lysis Buffer:50 mM Tris-HCl pH 7.4, 150 mM NaCl, 1mM EDTA, 1% Triton X-100, 0.1% SDS, 1% Sodium deoxycholate, 1mM PMSF.
Concentration
2 mg/ml
Quality Control Testing
Transient overexpression cell lysate was tested with Anti-CDC2L5 antibody (H00008621-M02) by Western Blots.
SDS-PAGE Gel
CDC2L5 transfected lysate
Western Blot
Lane 1: CDC2L5 transfected lysate ( 37 KDa).
Lane 2: Non-transfected lysate.Recommend Usage
Use it directly for immuno-precipitation, or heat lysate with SDS gel loading buffer to 95°C for 5 minutes followed by rapid cooling for western blot application. If dissociating conditions are required, add reducing agent prior to heating.
Storage Buffer
In modified RIPA Lysis Buffer.
Storage Instruction
Store at -80°C. Aliquot to avoid repeated freezing and thawing.
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Applications
Western Blot
Immunoprecipitation
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Gene Info — CDC2L5
Entrez GeneID
8621GeneBank Accession#
BC001274Protein Accession#
AAH01274Gene Name
CDC2L5
Gene Alias
CDC2L, CHED, FLJ35215, KIAA1791
Gene Description
cell division cycle 2-like 5 (cholinesterase-related cell division controller)
Omim ID
603309Gene Ontology
HyperlinkGene Summary
The protein encoded by this gene is a member of the cyclin-dependent serine/threonine protein kinase family. Members of this family are well known for their essential roles as master switches in cell cycle control. Some of the cell cycle control kinases are able to phosphorylate proteins that are important for cell differentiation and apoptosis, thus provide connections between cell proliferation, differentiation, and apoptosis. Proteins of this family may also be involved in non-cell cycle-related functions, such as neurocytoskeleton dynamics. The exact function of this protein has not yet been determined. It has unusually large N- and C-termini and is ubiquitously expressed in many tissues. Two alternatively spliced variants are described. [provided by RefSeq
Other Designations
CDC2-related protein kinase 5|cell division cycle 2-like 5|cell division cycle 2-like 5, isoform 1; cholinesterase-related cell division controller; CDC2-related protein kinase 5
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