Transient overexpression cell lysate was tested with Anti-AF5Q31 antibody (H00027125-M01) by Western Blots. SDS-PAGE Gel
AFF4 transfected lysate Western Blot
Lane 1: AFF4 transfected lysate ( 40 KDa). Lane 2: Non-transfected lysate.
Use it directly for immuno-precipitation, or heat lysate with SDS gel loading buffer to 95°C for 5 minutes followed by rapid cooling for western blot application. If dissociating conditions are required, add reducing agent prior to heating.
In modified RIPA Lysis Buffer.
Store at -80°C. Aliquot to avoid repeated freezing and thawing.