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IRAK4 HEK293 Cell Transient Overexpression Lysate(Non-Denatured)

  • Catalog # : L027T6
  • Visit Frequency :
  • Countries :
  • Specification
  • Transfected Cell Line:
  • HEK293
  • Plasmid:
  • pCMV-IRAK4 full length
  • Host:
  • Human
  • Theoretical MW (kDa):
  • 51.53
  • Lysis Buffer:
  • Modified RIPA Lysis Buffer:50 mM Tris-HCl pH 7.4, 150 mM NaCl, 1mM EDTA, 1% Triton X-100, 0.1% SDS, 1% Sodium deoxycholate, 1mM PMSF.
  • Concentration:
  • 2 mg/ml
  • Quality Control Testing:
  • Transient overexpression cell lysate was tested with Anti-IRAK4 antibody () by Western Blots.
    SDS-PAGE Gel
    QC Testing of L027T6
    IRAK4 transfected lysate
    Western Blot
    QC Testing of L027T6
    Lane 1: IRAK4 transfected lysate ( 51.53 KDa).
    Lane 2: Non-transfected lysate.
  • Recommend Usage:
  • Use it directly for immuno-precipitation, or heat lysate with SDS gel loading buffer to 95°C for 5 minutes followed by rapid cooling for western blot application. If dissociating conditions are required, add reducing agent prior to heating.
  • Storage Buffer:
  • In modified RIPA Lysis Buffer.
  • Storage Instruction:
  • Store at -80°C. Aliquot to avoid repeated freezing and thawing.
  • Applications
  • Western Blot
  • Application Image
  • Western Blot
  • Immunoprecipitation
  • Gene Information
  • Gene Name:
  • IRAK4
  • Gene Alias:
  • IPD1,NY-REN-64,REN64
  • Gene Description:
  • interleukin-1 receptor-associated kinase 4
  • Gene Summary:
  • This gene encodes a kinase that activates NF-kappaB in both the Toll-like receptor (TLR) and T-cell receptor (TCR) signaling pathways. The protein is essential for most innate immune responses. Mutations in this gene result in IRAK4 deficiency and recurrent invasive pneumococcal disease. Multiple transcript variants encoding different isoforms have been found for this gene. [supplied by RefSeq
  • Other Designations:
  • IRAK-4 mutated form 1,interleukin-1 receptor associated kinase 4
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