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BMPR1B HEK293 Cell Transient Overexpression Lysate(Non-Denatured)

  • Catalog # : L026T6
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  • Specification
  • Transfected Cell Line:
  • HEK293
  • Plasmid:
  • pCMV-BMPR1B full length
  • Host:
  • Human
  • Theoretical MW (kDa):
  • 57
  • Lysis Buffer:
  • Modified RIPA Lysis Buffer:50 mM Tris-HCl pH 7.4, 150 mM NaCl, 1mM EDTA, 1% Triton X-100, 0.1% SDS, 1% Sodium deoxycholate, 1mM PMSF.
  • Concentration:
  • 2 mg/ml
  • Quality Control Testing:
  • Transient overexpression cell lysate was tested with Anti-BMPR1B antibody (H00000658-M02) by Western Blots.
    SDS-PAGE Gel
    QC Testing of L026T6
    BMPR1B transfected lysate
    Western Blot
    QC Testing of L026T6
    Lane 1: BMPR1B transfected lysate ( 57 KDa).
    Lane 2: Non-transfected lysate.
  • Recommend Usage:
  • Use it directly for immuno-precipitation, or heat lysate with SDS gel loading buffer to 95°C for 5 minutes followed by rapid cooling for western blot application. If dissociating conditions are required, add reducing agent prior to heating.
  • Storage Buffer:
  • In modified RIPA Lysis Buffer.
  • Storage Instruction:
  • Store at -80°C. Aliquot to avoid repeated freezing and thawing.
  • Applications
  • Western Blot
  • Application Image
  • Western Blot
  • Immunoprecipitation
  • Gene Information
  • Entrez GeneID:
  • 658
  • Gene Name:
  • BMPR1B
  • Gene Alias:
  • ALK-6,ALK6,CDw293
  • Gene Description:
  • bone morphogenetic protein receptor, type IB
  • Gene Summary:
  • This gene encodes a member of the bone morphogenetic protein (BMP) receptor family of transmembrane serine/threonine kinases. The ligands of this receptor are BMPs, which are members of the TGF-beta superfamily. BMPs are involved in endochondral bone formation and embryogenesis. These proteins transduce their signals through the formation of heteromeric complexes of 2 different types of serine (threonine) kinase receptors: type I receptors of about 50-55 kD and type II receptors of about 70-80 kD. Type II receptors bind ligands in the absence of type I receptors, but they require their respective type I receptors for signaling, whereas type I receptors require their respective type II receptors for ligand binding. Mutations in this gene have been associated with primary pulmonary hypertension. [provided by RefSeq
  • Other Designations:
  • OTTHUMP00000161622,activin receptor-like kinase 6,serine/threonine receptor kinase
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