Transient overexpression cell lysate was tested with Anti-BCL7B antibody (H00009275-M01) by Western Blots. SDS-PAGE Gel
BCL7B transfected lysate Western Blot
Lane 1: BCL7B transfected lysate ( 22 KDa). Lane 2: Non-transfected lysate.
Use it directly for immuno-precipitation, or heat lysate with SDS gel loading buffer to 95°C for 5 minutes followed by rapid cooling for western blot application. If dissociating conditions are required, add reducing agent prior to heating.
In modified RIPA Lysis Buffer.
Store at -80°C. Aliquot to avoid repeated freezing and thawing.
The protein encoded by this gene contains a region that is highly similar to the N-terminal segment of BCL7A protein. The BCL7A protein is encoded by the gene known to be directly involved in a three-way gene translocation in a Burkitt lymphoma cell line. This gene is located at a chromosomal region commonly deleted in Williams syndrome. The function of this gene has not yet been determined. [provided by RefSeq