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BCL7B HEK293 Cell Transient Overexpression Lysate(Non-Denatured)

  • Catalog # : L025T6
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  • Specification
  • Transfected Cell Line:
  • HEK293
  • Plasmid:
  • pCMV-BCL7B full length
  • Host:
  • Human
  • Theoretical MW (kDa):
  • 22
  • Lysis Buffer:
  • Modified RIPA Lysis Buffer:50 mM Tris-HCl pH 7.4, 150 mM NaCl, 1mM EDTA, 1% Triton X-100, 0.1% SDS, 1% Sodium deoxycholate, 1mM PMSF.
  • Concentration:
  • 2 mg/ml
  • Quality Control Testing:
  • Transient overexpression cell lysate was tested with Anti-BCL7B antibody (H00009275-M01) by Western Blots.
    SDS-PAGE Gel
    QC Testing of L025T6
    BCL7B transfected lysate
    Western Blot
    QC Testing of L025T6
    Lane 1: BCL7B transfected lysate ( 22 KDa).
    Lane 2: Non-transfected lysate.
  • Recommend Usage:
  • Use it directly for immuno-precipitation, or heat lysate with SDS gel loading buffer to 95°C for 5 minutes followed by rapid cooling for western blot application. If dissociating conditions are required, add reducing agent prior to heating.
  • Storage Buffer:
  • In modified RIPA Lysis Buffer.
  • Storage Instruction:
  • Store at -80°C. Aliquot to avoid repeated freezing and thawing.
  • Applications
  • Western Blot
  • Application Image
  • Western Blot
  • Immunoprecipitation
  • Gene Information
  • Entrez GeneID:
  • 9275
  • Gene Name:
  • BCL7B
  • Gene Alias:
  • -
  • Gene Description:
  • B-cell CLL/lymphoma 7B
  • Gene Summary:
  • The protein encoded by this gene contains a region that is highly similar to the N-terminal segment of BCL7A protein. The BCL7A protein is encoded by the gene known to be directly involved in a three-way gene translocation in a Burkitt lymphoma cell line. This gene is located at a chromosomal region commonly deleted in Williams syndrome. The function of this gene has not yet been determined. [provided by RefSeq
  • Other Designations:
  • OTTHUMP00000025030
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