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ADA HEK293 Cell Transient Overexpression Lysate(Non-Denatured)

  • Catalog # : L024T6
  • Visit Frequency :
  • Countries :
  • Specification
  • Transfected Cell Line:
  • HEK293
  • Plasmid:
  • pCMV-ADA full length
  • Host:
  • Human
  • Theoretical MW (kDa):
  • 41
  • Lysis Buffer:
  • Modified RIPA Lysis Buffer:50 mM Tris-HCl pH 7.4, 150 mM NaCl, 1mM EDTA, 1% Triton X-100, 0.1% SDS, 1% Sodium deoxycholate, 1mM PMSF.
  • Concentration:
  • 2 mg/ml
  • Quality Control Testing:
  • Transient overexpression cell lysate was tested with Anti-ADA antibody (H00000100-M01) by Western Blots.
    SDS-PAGE Gel
    QC Testing of L024T6
    ADA transfected lysate
    Western Blot
    QC Testing of L024T6
    Lane 1: ADA transfected lysate ( 41 KDa).
    Lane 2: Non-transfected lysate.
  • Recommend Usage:
  • Use it directly for immuno-precipitation, or heat lysate with SDS gel loading buffer to 95°C for 5 minutes followed by rapid cooling for western blot application. If dissociating conditions are required, add reducing agent prior to heating.
  • Storage Buffer:
  • In modified RIPA Lysis Buffer.
  • Storage Instruction:
  • Store at -80°C. Aliquot to avoid repeated freezing and thawing.
  • Applications
  • Western Blot
  • Application Image
  • Western Blot
  • Immunoprecipitation
  • Gene Information
  • Entrez GeneID:
  • 100
  • Gene Name:
  • ADA
  • Gene Alias:
  • -
  • Gene Description:
  • adenosine deaminase
  • Gene Summary:
  • This gene encodes an enzyme that catalyzes the hydrolysis of adenosine to inosine. Various mutations have been described for this gene and have been linked to human diseases. Deficiency in this enzyme causes a form of severe combined immunodeficiency disease (SCID), in which there is dysfunction of both B and T lymphocytes with impaired cellular immunity and decreased production of immunoglobulins, whereas elevated levels of this enzyme have been associated with congenital hemolytic anemia. [provided by RefSeq
  • Other Designations:
  • adenosine aminohydrolase
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