ACOX2 HEK293 Cell Transient Overexpression Lysate(Non-Denatured)
* The price is valid only in USA. Please select country.
-
More Files
- More Functions
-
Specification
Transfected Cell Line
HEK293
Plasmid
pCMV-ACOX2 full length
Host
Human
Theoretical MW (kDa)
77
Lysis Buffer
Modified RIPA Lysis Buffer:50 mM Tris-HCl pH 7.4, 150 mM NaCl, 1mM EDTA, 1% Triton X-100, 0.1% SDS, 1% Sodium deoxycholate, 1mM PMSF.
Concentration
2 mg/ml
Quality Control Testing
Transient overexpression cell lysate was tested with Anti-ACOX2 antibody (H00008309-M01) by Western Blots.
SDS-PAGE Gel
ACOX2 transfected lysate
Western Blot
Lane 1: ACOX2 transfected lysate ( 77 KDa).
Lane 2: Non-transfected lysate.Recommend Usage
Use it directly for immuno-precipitation, or heat lysate with SDS gel loading buffer to 95°C for 5 minutes followed by rapid cooling for western blot application. If dissociating conditions are required, add reducing agent prior to heating.
Storage Buffer
In modified RIPA Lysis Buffer.
Storage Instruction
Store at -80°C. Aliquot to avoid repeated freezing and thawing.
-
Applications
Western Blot
Immunoprecipitation
-
Gene Info — ACOX2
Entrez GeneID
8309GeneBank Accession#
NM_003500Protein Accession#
NP_003491Gene Name
ACOX2
Gene Alias
BCOX, BRCACOX, BRCOX, THCCox
Gene Description
acyl-Coenzyme A oxidase 2, branched chain
Omim ID
601641Gene Ontology
HyperlinkGene Summary
The product of this gene belongs to the acyl-CoA oxidase family. It encodes the branched-chain acyl-CoA oxidase which is involved in the degradation of long branched fatty acids and bile acid intermediates in peroxisomes. Deficiency of this enzyme results in the accumulation of branched fatty acids and bile acid intermediates, and may lead to Zellweger syndrome, severe mental retardation, and death in children. [provided by RefSeq
Other Designations
Peroxisomal branched chain acyl-CoA oxidase|THCA-CoA oxidase|Trihydroxycoprostanoyl-CoA oxidase
-
Interactome
-
Pathway
-
Disease
- +1-909-264-1399
+1-909-992-0619
Toll Free : +1-877-853-6098 - +1-909-992-3401
- sales@abnova.com