ARH HEK293 Cell Transient Overexpression Lysate(Non-Denatured)
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Specification
Transfected Cell Line
HEK293
Plasmid
pCMV-ARH full length
Host
Human
Theoretical MW (kDa)
29
Lysis Buffer
Modified RIPA Lysis Buffer:50 mM Tris-HCl pH 7.4, 150 mM NaCl, 1mM EDTA, 1% Triton X-100, 0.1% SDS, 1% Sodium deoxycholate, 1mM PMSF.
Concentration
2 mg/ml
Quality Control Testing
Transient overexpression cell lysate was tested with Anti-ARH antibody (H00026119-M01) by Western Blots.
SDS-PAGE Gel
LDLRAP1 transfected lysate
Western Blot
Lane 1: LDLRAP1 transfected lysate ( 29 KDa).
Lane 2: Non-transfected lysate.Recommend Usage
Use it directly for immuno-precipitation, or heat lysate with SDS gel loading buffer to 95°C for 5 minutes followed by rapid cooling for western blot application. If dissociating conditions are required, add reducing agent prior to heating.
Storage Buffer
In modified RIPA Lysis Buffer.
Storage Instruction
Store at -80°C. Aliquot to avoid repeated freezing and thawing.
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Applications
Western Blot
Immunoprecipitation
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Gene Info — LDLRAP1
Entrez GeneID
26119GeneBank Accession#
BC029770Protein Accession#
AAH29770Gene Name
LDLRAP1
Gene Alias
ARH, ARH1, ARH2, DKFZp586D0624, FHCB1, FHCB2, MGC34705
Gene Description
low density lipoprotein receptor adaptor protein 1
Gene Ontology
HyperlinkGene Summary
The protein encoded by this gene is a cytosolic protein which contains a phosphotyrosine binding (PTD) domain. The PTD domain has been found to interact with the cytoplasmic tail of the LDL receptor. Mutations in this gene lead to LDL receptor malfunction and cause the disorder autosomal recessive hypercholesterolaemia. [provided by RefSeq
Other Designations
LDL receptor adaptor protein|OTTHUMP00000008526|autosomal recessive hypercholesterolemia protein
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Interactome
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Disease
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