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ARH HEK293 Cell Transient Overexpression Lysate(Non-Denatured)

  • Catalog # : L019T6
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  • Specification
  • Transfected Cell Line:
  • HEK293
  • Plasmid:
  • pCMV-ARH full length
  • Host:
  • Human
  • Theoretical MW (kDa):
  • 29
  • Lysis Buffer:
  • Modified RIPA Lysis Buffer:50 mM Tris-HCl pH 7.4, 150 mM NaCl, 1mM EDTA, 1% Triton X-100, 0.1% SDS, 1% Sodium deoxycholate, 1mM PMSF.
  • Concentration:
  • 2 mg/ml
  • Quality Control Testing:
  • Transient overexpression cell lysate was tested with Anti-ARH antibody (H00026119-M01) by Western Blots.
    SDS-PAGE Gel
    QC Testing of L019T6
    LDLRAP1 transfected lysate
    Western Blot
    QC Testing of L019T6
    Lane 1: LDLRAP1 transfected lysate ( 29 KDa).
    Lane 2: Non-transfected lysate.
  • Recommend Usage:
  • Use it directly for immuno-precipitation, or heat lysate with SDS gel loading buffer to 95°C for 5 minutes followed by rapid cooling for western blot application. If dissociating conditions are required, add reducing agent prior to heating.
  • Storage Buffer:
  • In modified RIPA Lysis Buffer.
  • Storage Instruction:
  • Store at -80°C. Aliquot to avoid repeated freezing and thawing.
  • Applications
  • Western Blot
  • Application Image
  • Western Blot
  • Immunoprecipitation
  • Gene Information
  • Gene Name:
  • LDLRAP1
  • Gene Alias:
  • ARH,ARH1,ARH2,DKFZp586D0624,FHCB1,FHCB2,MGC34705
  • Gene Description:
  • low density lipoprotein receptor adaptor protein 1
  • Gene Summary:
  • The protein encoded by this gene is a cytosolic protein which contains a phosphotyrosine binding (PTD) domain. The PTD domain has been found to interact with the cytoplasmic tail of the LDL receptor. Mutations in this gene lead to LDL receptor malfunction and cause the disorder autosomal recessive hypercholesterolaemia. [provided by RefSeq
  • Other Designations:
  • LDL receptor adaptor protein,OTTHUMP00000008526,autosomal recessive hypercholesterolemia protein
  • Gene Pathway
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