Product Browser

Last updated: 2016/12/4

Product Compare

Product Compare Cancel Click this icon to add products to compare list. Select up to 10 products.

Quick Order (Tutorial)

Input Catalog #,
place order here!
Catalog # :
  • Where to buy
  • Choose your location

NIH/3T3 (mouse NIH/Swiss embryo) whole cell lysate (denatured)

  • Catalog # : L018V1
  • Visit Frequency :
  • Countries :
  • Specification
  • Product Description:
  • Whole cell lysate (denatured)
  • Tissue:
  • Embryo, Contact-Inhibited
  • Host:
  • Mouse
  • Preparation Method:
  • Cell lysate was prepared by homogenization in ice-cold modified RIPA Lysis Buffer with cocktail of protease inhibitors (Sigma). Cell debris was removed by centrifugation. Protein concentration was determined with Bradford assay (Bio-Rad protein assay, Microplate Standard assay). The lysate was mixed in 5X Sample Buffer to become final 1X Sample Buffer as Storage Buffer. The lysate was heated at 95°C for 5 min, and cooled rapidly.
  • Lysis Buffer:
  • Modified RIPA Lysis Buffer: 50 mM Tris-HCl pH 7.4, 150 mM NaCl, 1mM EDTA, 1% Triton X-100, 0.1% SDS, 1% Sodium deoxycholate, 1mM PMSF.
  • Quality Control Testing:
  • 12.5% SDS-PAGE Stained with Coomassie Blue.
    SDS-PAGE Gel
    QC Testing of L018V1
  • Recommend Usage:
  • The lysate is ready to load on SDS-PAGE for Western blotting.
  • Storage Buffer:
  • In ready-to-use 1X Sample Buffer (50 mM Tris-HCl, 2% SDS, 10% glycerol, 300 mM 2-mercaptoethanol, 0.01% Bromophenol blue).
  • Storage Instruction:
  • Store at -80°C. Aliquot to avoid repeated freezing and thawing.
  • Applications
  • Western Blot
  • Application Image
  • Western Blot
  • RSS
  • YouTube
  • Linkedin
  • Facebook