ANXA2 HEK293 Cell Transient Overexpression Lysate(Non-Denatured)
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Specification
Transfected Cell Line
HEK293
Plasmid
pCMV-ANXA2 full length
Host
Human
Theoretical MW (kDa)
39
Lysis Buffer
Modified RIPA Lysis Buffer:50 mM Tris-HCl pH 7.4, 150 mM NaCl, 1mM EDTA, 1% Triton X-100, 0.1% SDS, 1% Sodium deoxycholate, 1mM PMSF.
Concentration
2 mg/ml
Quality Control Testing
Transient overexpression cell lysate was tested with Anti-ANXA2 antibody (H00000302-M02) by Western Blots.
SDS-PAGE Gel
ANXA2 transfected lysate
Western Blot
Lane 1: ANXA2 transfected lysate ( 39 KDa).
Lane 2: Non-transfected lysate.Recommend Usage
Use it directly for immuno-precipitation, or heat lysate with SDS gel loading buffer to 95°C for 5 minutes followed by rapid cooling for western blot application. If dissociating conditions are required, add reducing agent prior to heating.
Storage Buffer
In modified RIPA Lysis Buffer.
Storage Instruction
Store at -80°C. Aliquot to avoid repeated freezing and thawing.
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Applications
Western Blot
Immunoprecipitation
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Gene Info — ANXA2
Entrez GeneID
302GeneBank Accession#
BC009564Protein Accession#
AAH09564Gene Name
ANXA2
Gene Alias
ANX2, ANX2L4, CAL1H, LIP2, LPC2, LPC2D, P36, PAP-IV
Gene Description
annexin A2
Omim ID
151740Gene Ontology
HyperlinkGene Summary
This gene encodes a member of the annexin family. Members of this calcium-dependent phospholipid-binding protein family play a role in the regulation of cellular growth and in signal transduction pathways. This protein functions as an autocrine factor which heightens osteoclast formation and bone resorption. This gene has three pseudogenes located on chromosomes 4, 9 and 10, respectively. Multiple alternatively spliced transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq
Other Designations
OTTHUMP00000163650|annexin II|calpactin I heavy polypeptide|chromobindin 8|lipocortin II|placental anticoagulant protein IV
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