Transient overexpression cell lysate was tested with Anti-ANXA2 antibody (H00000302-M02) by Western Blots. SDS-PAGE Gel
ANXA2 transfected lysate Western Blot
Lane 1: ANXA2 transfected lysate ( 39 KDa). Lane 2: Non-transfected lysate.
Use it directly for immuno-precipitation, or heat lysate with SDS gel loading buffer to 95°C for 5 minutes followed by rapid cooling for western blot application. If dissociating conditions are required, add reducing agent prior to heating.
In modified RIPA Lysis Buffer.
Store at -80°C. Aliquot to avoid repeated freezing and thawing.
This gene encodes a member of the annexin family. Members of this calcium-dependent phospholipid-binding protein family play a role in the regulation of cellular growth and in signal transduction pathways. This protein functions as an autocrine factor which heightens osteoclast formation and bone resorption. This gene has three pseudogenes located on chromosomes 4, 9 and 10, respectively. Multiple alternatively spliced transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq
OTTHUMP00000163650,annexin II,calpactin I heavy polypeptide,chromobindin 8,lipocortin II,placental anticoagulant protein IV