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ANXA2 HEK293 Cell Transient Overexpression Lysate(Non-Denatured)

  • Catalog # : L016T6
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  • Specification
  • Transfected Cell Line:
  • HEK293
  • Plasmid:
  • pCMV-ANXA2 full length
  • Host:
  • Human
  • Theoretical MW (kDa):
  • 39
  • Lysis Buffer:
  • Modified RIPA Lysis Buffer:50 mM Tris-HCl pH 7.4, 150 mM NaCl, 1mM EDTA, 1% Triton X-100, 0.1% SDS, 1% Sodium deoxycholate, 1mM PMSF.
  • Concentration:
  • 2 mg/ml
  • Quality Control Testing:
  • Transient overexpression cell lysate was tested with Anti-ANXA2 antibody (H00000302-M02) by Western Blots.
    SDS-PAGE Gel
    QC Testing of L016T6
    ANXA2 transfected lysate
    Western Blot
    QC Testing of L016T6
    Lane 1: ANXA2 transfected lysate ( 39 KDa).
    Lane 2: Non-transfected lysate.
  • Recommend Usage:
  • Use it directly for immuno-precipitation, or heat lysate with SDS gel loading buffer to 95°C for 5 minutes followed by rapid cooling for western blot application. If dissociating conditions are required, add reducing agent prior to heating.
  • Storage Buffer:
  • In modified RIPA Lysis Buffer.
  • Storage Instruction:
  • Store at -80°C. Aliquot to avoid repeated freezing and thawing.
  • Applications
  • Western Blot
  • Application Image
  • Western Blot
  • Immunoprecipitation
  • Gene Information
  • Entrez GeneID:
  • 302
  • Gene Name:
  • ANXA2
  • Gene Alias:
  • ANX2,ANX2L4,CAL1H,LIP2,LPC2,LPC2D,P36,PAP-IV
  • Gene Description:
  • annexin A2
  • Gene Summary:
  • This gene encodes a member of the annexin family. Members of this calcium-dependent phospholipid-binding protein family play a role in the regulation of cellular growth and in signal transduction pathways. This protein functions as an autocrine factor which heightens osteoclast formation and bone resorption. This gene has three pseudogenes located on chromosomes 4, 9 and 10, respectively. Multiple alternatively spliced transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq
  • Other Designations:
  • OTTHUMP00000163650,annexin II,calpactin I heavy polypeptide,chromobindin 8,lipocortin II,placental anticoagulant protein IV
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