A-431 (human epidermoid carcinoma) whole cell lysate (non-denatured)
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Specifications
Product Description
Whole cell lysate (non-denatured).
Tissue
Epidermis
Host
Human
Preparation Method
Cell lysate was prepared by homogenization in ice-cold modified RIPA Lysis Buffer with cocktail of protease inhibitors (Sigma). Cell debris was removed by centrifugation. Protein concentration was determined with Bradford assay (Bio-Rad protein assay, Microplate Standard assay).
Lysis Buffer
Modified RIPA Lysis Buffer: 50 mM Tris-HCl pH 7.4, 150 mM NaCl, 1mM EDTA, 1% Triton X-100, 0.1% SDS, 1% Sodium deoxycholate, 1mM PMSF.
Quality Control Testing
12.5% SDS-PAGE Stained with Coomassie Blue.
SDS-PAGE Gel
Recommend Usage
Use it directly for immuno-precipitation, or heat lysate with SDS gel loading buffer to 95°C for 5 minutes followed by rapid cooling for western blot application. If dissociating conditions are required, add reducing agent prior to heating.
Storage Buffer
In modified RIPA Lysis Buffer.
Storage Instruction
Store at -80°C. Aliquot to avoid repeated freezing and thawing.
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Applications
Western Blot
Immunoprecipitation
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Publication Reference
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Microfluidic communicating vessel chip for expedited and automated immunomagnetic assays.
Yang Y, Zeng Y.
Lab on a Chip 2018 Dec; 18(24):3830.
Application:Microchip Immunomagnetic Assay, Human, A431 cells.
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Microfluidic communicating vessel chip for expedited and automated immunomagnetic assays.
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