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ALS2 HEK293 Cell Transient Overexpression Lysate(Non-Denatured)

  • Catalog # : L013T6
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  • Specification
  • Transfected Cell Line:
  • HEK293
  • Plasmid:
  • pCMV-ALS2 full length
  • Host:
  • Human
  • Theoretical MW (kDa):
  • 184
  • Lysis Buffer:
  • Modified RIPA Lysis Buffer:50 mM Tris-HCl pH 7.4, 150 mM NaCl, 1mM EDTA, 1% Triton X-100, 0.1% SDS, 1% Sodium deoxycholate, 1mM PMSF.
  • Concentration:
  • 2 mg/ml
  • Quality Control Testing:
  • Transient overexpression cell lysate was tested with Anti-ALS2 antibody (H00057679-M01) by Western Blots.
    SDS-PAGE Gel
    QC Testing of L013T6
    ALS2 transfected lysate
    Western Blot
    QC Testing of L013T6
    Lane 1: ALS2 transfected lysate ( 184 KDa).
    Lane 2: Non-transfected lysate.
  • Recommend Usage:
  • Use it directly for immuno-precipitation, or heat lysate with SDS gel loading buffer to 95°C for 5 minutes followed by rapid cooling for western blot application. If dissociating conditions are required, add reducing agent prior to heating.
  • Storage Buffer:
  • In modified RIPA Lysis Buffer.
  • Storage Instruction:
  • Store at -80°C. Aliquot to avoid repeated freezing and thawing.
  • Applications
  • Western Blot
  • Application Image
  • Western Blot
  • Immunoprecipitation
  • Gene Information
  • Gene Name:
  • ALS2
  • Gene Alias:
  • ALS2CR6,ALSJ,FLJ31851,IAHSP,KIAA1563,MGC87187,PLSJ
  • Gene Description:
  • amyotrophic lateral sclerosis 2 (juvenile)
  • Gene Summary:
  • The protein encoded by this gene contains an ATS1/RCC1-like domain, a RhoGEF domain, and a vacuolar protein sorting 9 (VPS9) domain, all of which are guanine-nucleotide exchange factors that activate members of the Ras superfamily of GTPases. The protein functions as a guanine nucleotide exchange factor for the small GTPase RAB5. The protein localizes with RAB5 on early endosomal compartments, and functions as a modulator for endosomal dynamics. Mutations in this gene result in several forms of juvenile lateral sclerosis and infantile-onset ascending spastic paralysis. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq
  • Other Designations:
  • alsin
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