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Last updated: 2017/1/15
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ALDOA HEK293 Cell Transient Overexpression Lysate(Non-Denatured)

  • Catalog # : L012T6
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  • Specification
  • Transfected Cell Line:
  • HEK293
  • Plasmid:
  • pCMV-ALDOA full length
  • Host:
  • Human
  • Theoretical MW (kDa):
  • 39
  • Lysis Buffer:
  • Modified RIPA Lysis Buffer:50 mM Tris-HCl pH 7.4, 150 mM NaCl, 1mM EDTA, 1% Triton X-100, 0.1% SDS, 1% Sodium deoxycholate, 1mM PMSF.
  • Concentration:
  • 2 mg/ml
  • Quality Control Testing:
  • Transient overexpression cell lysate was tested with Anti-ALDOA antibody (H00000226-M02) by Western Blots.
    SDS-PAGE Gel
    QC Testing of L012T6
    ALDOA transfected lysate
    Western Blot
    QC Testing of L012T6
    Lane 1: ALDOA transfected lysate ( 39 KDa).
    Lane 2: Non-transfected lysate.
  • Recommend Usage:
  • Use it directly for immuno-precipitation, or heat lysate with SDS gel loading buffer to 95°C for 5 minutes followed by rapid cooling for western blot application. If dissociating conditions are required, add reducing agent prior to heating.
  • Storage Buffer:
  • In modified RIPA Lysis Buffer.
  • Storage Instruction:
  • Store at -80°C. Aliquot to avoid repeated freezing and thawing.
  • Applications
  • Western Blot
  • Application Image
  • Western Blot
  • Immunoprecipitation
  • Gene Information
  • Entrez GeneID:
  • 226
  • Gene Name:
  • ALDOA
  • Gene Alias:
  • ALDA,MGC10942,MGC17716,MGC17767
  • Gene Description:
  • aldolase A, fructose-bisphosphate
  • Gene Summary:
  • This gene product, Aldolase A (fructose-bisphosphate aldolase) is a glycolytic enzyme that catalyzes the reversible conversion of fructose-1,6-bisphosphate to glyceraldehyde 3-phosphate and dihydroxyacetone phosphate. Three aldolase isozymes (A, B, and C), encoded by three different genes, are differentially expressed during development. Aldolase A is found in the developing embryo and is produced in even greater amounts in adult muscle. Aldolase A expression is repressed in adult liver, kidney and intestine and similar to aldolase C levels in brain and other nervous tissue. Aldolase A deficiency has been associated with myopathy and hemolytic anemia. Alternative splicing of this gene results in multiple transcript variants which encode the same protein. [provided by RefSeq
  • Other Designations:
  • aldolase A,fructose-1,6-bisphosphate triosephosphate-lyase,fructose-bisphosphate aldolase A
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