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Last updated: 2017/3/26
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ALAS2 HEK293 Cell Transient Overexpression Lysate(Non-Denatured)

  • Catalog # : L011T6
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  • Specification
  • Transfected Cell Line:
  • HEK293
  • Plasmid:
  • pCMV-ALAS2 full length
  • Host:
  • Human
  • Theoretical MW (kDa):
  • 64
  • Lysis Buffer:
  • Modified RIPA Lysis Buffer:50 mM Tris-HCl pH 7.4, 150 mM NaCl, 1mM EDTA, 1% Triton X-100, 0.1% SDS, 1% Sodium deoxycholate, 1mM PMSF.
  • Concentration:
  • 2 mg/ml
  • Quality Control Testing:
  • Transient overexpression cell lysate was tested with Anti-ALAS2 antibody (H00000212-M01) by Western Blots.
    SDS-PAGE Gel
    QC Testing of L011T6
    ALAS2 transfected lysate
    Western Blot
    QC Testing of L011T6
    Lane 1: ALAS2 transfected lysate ( 64 KDa).
    Lane 2: Non-transfected lysate.
  • Recommend Usage:
  • Use it directly for immuno-precipitation, or heat lysate with SDS gel loading buffer to 95°C for 5 minutes followed by rapid cooling for western blot application. If dissociating conditions are required, add reducing agent prior to heating.
  • Storage Buffer:
  • In modified RIPA Lysis Buffer.
  • Storage Instruction:
  • Store at -80°C. Aliquot to avoid repeated freezing and thawing.
  • Applications
  • Western Blot
  • Application Image
  • Western Blot
  • Immunoprecipitation
  • Gene Information
  • Entrez GeneID:
  • 212
  • Gene Name:
  • ALAS2
  • Gene Alias:
  • ALAS-E,ALASE,ANH1,ASB,FLJ93603,XLSA
  • Gene Description:
  • aminolevulinate, delta-, synthase 2
  • Gene Summary:
  • The product of this gene specifies an erythroid-specific mitochondrially located enzyme. The encoded protein catalyzes the first step in the heme biosynthetic pathway. Defects in this gene cause X-linked pyridoxine-responsive sideroblastic anemia. Alternatively spliced transcript variants encoding different isoforms have been identified. [provided by RefSeq
  • Other Designations:
  • 5-aminolevulinate synthase, erythroid-specific, mitochondrial,5-aminolevulinic acid synthase,OTTHUMP00000023388,OTTHUMP00000023389,delta-ALA synthetase,delta-aminolevulinate synthase
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