ALAS2 HEK293 Cell Transient Overexpression Lysate(Non-Denatured)
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Specification
Transfected Cell Line
HEK293
Plasmid
pCMV-ALAS2 full length
Host
Human
Theoretical MW (kDa)
64
Lysis Buffer
Modified RIPA Lysis Buffer:50 mM Tris-HCl pH 7.4, 150 mM NaCl, 1mM EDTA, 1% Triton X-100, 0.1% SDS, 1% Sodium deoxycholate, 1mM PMSF.
Concentration
2 mg/ml
Quality Control Testing
Transient overexpression cell lysate was tested with Anti-ALAS2 antibody (H00000212-M01) by Western Blots.
SDS-PAGE Gel
ALAS2 transfected lysate
Western Blot
Lane 1: ALAS2 transfected lysate ( 64 KDa).
Lane 2: Non-transfected lysate.Recommend Usage
Use it directly for immuno-precipitation, or heat lysate with SDS gel loading buffer to 95°C for 5 minutes followed by rapid cooling for western blot application. If dissociating conditions are required, add reducing agent prior to heating.
Storage Buffer
In modified RIPA Lysis Buffer.
Storage Instruction
Store at -80°C. Aliquot to avoid repeated freezing and thawing.
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Applications
Western Blot
Immunoprecipitation
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Gene Info — ALAS2
Entrez GeneID
212GeneBank Accession#
NM_000032Protein Accession#
NP_000023Gene Name
ALAS2
Gene Alias
ALAS-E, ALASE, ANH1, ASB, FLJ93603, XLSA
Gene Description
aminolevulinate, delta-, synthase 2
Omim ID
301300Gene Ontology
HyperlinkGene Summary
The product of this gene specifies an erythroid-specific mitochondrially located enzyme. The encoded protein catalyzes the first step in the heme biosynthetic pathway. Defects in this gene cause X-linked pyridoxine-responsive sideroblastic anemia. Alternatively spliced transcript variants encoding different isoforms have been identified. [provided by RefSeq
Other Designations
5-aminolevulinate synthase, erythroid-specific, mitochondrial|5-aminolevulinic acid synthase|OTTHUMP00000023388|OTTHUMP00000023389|delta-ALA synthetase|delta-aminolevulinate synthase
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