AHCYL1 HEK293 Cell Transient Overexpression Lysate(Non-Denatured)
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More Files
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Specification
Transfected Cell Line
HEK293
Plasmid
pCMV-AHCYL1 full length
Host
Human
Theoretical MW (kDa)
69
Lysis Buffer
Modified RIPA Lysis Buffer:50 mM Tris-HCl pH 7.4, 150 mM NaCl, 1mM EDTA, 1% Triton X-100, 0.1% SDS, 1% Sodium deoxycholate, 1mM PMSF.
Concentration
2 mg/ml
Quality Control Testing
Transient overexpression cell lysate was tested with Anti-AHCYL1 antibody (H00010768-M05) by Western Blots.
SDS-PAGE Gel
AHCYL1 transfected lysate
Western Blot
Lane 1: AHCYL1 transfected lysate ( 69 KDa).
Lane 2: Non-transfected lysate.Recommend Usage
Use it directly for immuno-precipitation, or heat lysate with SDS gel loading buffer to 95°C for 5 minutes followed by rapid cooling for western blot application. If dissociating conditions are required, add reducing agent prior to heating.
Storage Buffer
In modified RIPA Lysis Buffer.
Storage Instruction
Store at -80°C. Aliquot to avoid repeated freezing and thawing.
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Applications
Western Blot
Immunoprecipitation
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Gene Info — AHCYL1
Entrez GeneID
10768GeneBank Accession#
NM_006621Protein Accession#
NP_006612Gene Name
AHCYL1
Gene Alias
DCAL, IRBIT, PRO0233, XPVKONA
Gene Description
S-adenosylhomocysteine hydrolase-like 1
Omim ID
607826Gene Ontology
HyperlinkGene Summary
4
Other Designations
OTTHUMP00000013376|S-adenosyl homocysteine hydrolase homolog|dendritic cell expressed AHCY-like protein|inositol 1,4,5-trisphosphate receptor-binding protein
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