NKX2-5 HEK293 Cell Transient Overexpression Lysate(Non-Denatured)
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More Files
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SDS-PAGE Gel
NKX2-5 transfected lysate
Western Blot
Lane 1: NKX2-5 transfected lysate ( 34.92 KDa).
Lane 2: Non-transfected lysate.
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Specification
Transfected Cell Line
HEK293
Plasmid
pCMV-NKX2-5 full length
Host
Human
Theoretical MW (kDa)
34.92
Lysis Buffer
Modified RIPA Lysis Buffer:50 mM Tris-HCl pH 7.4, 150 mM NaCl, 1mM EDTA, 1% Triton X-100, 0.1% SDS, 1% Sodium deoxycholate, 1mM PMSF.
Concentration
2 mg/ml
Quality Control Testing
Transient overexpression cell lysate was tested with Anti-NKX2-5 antibody (H00001482-M03 ) by Western Blots.
SDS-PAGE Gel
NKX2-5 transfected lysate
Western Blot
Lane 1: NKX2-5 transfected lysate ( 34.92 KDa).
Lane 2: Non-transfected lysate.Recommend Usage
Use it directly for immuno-precipitation, or heat lysate with SDS gel loading buffer to 95°C for 5 minutes followed by rapid cooling for western blot application. If dissociating conditions are required, add reducing agent prior to heating.
Storage Buffer
In modified RIPA Lysis Buffer.
Storage Instruction
Store at -80°C. Aliquot to avoid repeated freezing and thawing.
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Applications
Western Blot
Immunoprecipitation
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Gene Info — NKX2-5
Entrez GeneID
1482GeneBank Accession#
BC025711Protein Accession#
AAH25711Gene Name
NKX2-5
Gene Alias
CHNG5, CSX, CSX1, NKX2.5, NKX2E, NKX4-1
Gene Description
NK2 transcription factor related, locus 5 (Drosophila)
Gene Ontology
HyperlinkGene Summary
Homeobox-containing genes play critical roles in regulating tissue-specific gene expression essential for tissue differentiation, as well as determining the temporal and spatial patterns of development (Shiojima et al., 1995 [PubMed 7665173]). It has been demonstrated that a Drosophila homeobox-containing gene called 'tinman' is expressed in the developing dorsal vessel and in the equivalent of the vertebrate heart. Mutations in tinman result in loss of heart formation in the embryo, suggesting that tinman is essential for Drosophila heart formation. Furthermore, abundant expression of Csx, the presumptive mouse homolog of tinman, is observed only in the heart from the time of cardiac differentiation. CSX, the human homolog of murine Csx, has a homeodomain sequence identical to that of Csx and is expressed only in the heart, again suggesting that CSX plays an important role in human heart formation.[supplied by OMIM
Other Designations
NK2 transcription factor homolog E|NK2 transcription factor related, locus 5|cardiac-specific homeo box|tinman paralog
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