OLIG1 HEK293 Cell Transient Overexpression Lysate(Non-Denatured)
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More Files
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Specification
Transfected Cell Line
HEK293
Plasmid
pCMV-OLIG1 full length
Host
Human
Theoretical MW (kDa)
27.905
Lysis Buffer
Modified RIPA Lysis Buffer:50 mM Tris-HCl pH 7.4, 150 mM NaCl, 1mM EDTA, 1% Triton X-100, 0.1% SDS, 1% Sodium deoxycholate, 1mM PMSF.
Concentration
2 mg/ml
Quality Control Testing
Transient overexpression cell lysate was tested with Anti-OLIG1 antibody by Western Blots.
SDS-PAGE Gel
OLIG1 transfected lysate
Western Blot
Lane 1: OLIG1 transfected lysate ( 27.905 KDa).
Lane 2: Non-transfected lysate.Recommend Usage
Use it directly for immuno-precipitation, or heat lysate with SDS gel loading buffer to 95°C for 5 minutes followed by rapid cooling for western blot application. If dissociating conditions are required, add reducing agent prior to heating.
Storage Buffer
In modified RIPA Lysis Buffer.
Storage Instruction
Store at -80°C. Aliquot to avoid repeated freezing and thawing.
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Applications
Western Blot
Immunoprecipitation
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Gene Info — OLIG1
Entrez GeneID
116448GeneBank Accession#
NM_138983.1Protein Accession#
NP_620450.1Gene Name
OLIG1
Gene Alias
BHLHB6, bHLHe21
Gene Description
oligodendrocyte transcription factor 1
Omim ID
606385Gene Ontology
HyperlinkGene Summary
helix-loop-helix protein
Other Designations
basic domain, helix-loop-helix protein, class B, 6|oligodendrocyte lineage transcription factor 1|oligodendrocyte-specific bHLH transcription factor 1
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