ZSCAN21 HEK293 Cell Transient Overexpression Lysate(Non-Denatured)
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More Files
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Specification
Transfected Cell Line
HEK293
Plasmid
pCMV-ZSCAN21 full length
Host
Human
Theoretical MW (kDa)
50.9
Lysis Buffer
Modified RIPA Lysis Buffer:50 mM Tris-HCl pH 7.4, 150 mM NaCl, 1mM EDTA, 1% Triton X-100, 0.1% SDS, 1% Sodium deoxycholate, 1mM PMSF.
Concentration
2 mg/ml
Quality Control Testing
Transient overexpression cell lysate was tested with Anti-ZSCAN21 antibody (H00007589-M18) by Western Blots.
SDS-PAGE Gel
ZSCAN21 transfected lysate
Western Blot
Lane 1: ZSCAN21 transfected lysate ( 50.9 KDa).
Lane 2: Non-transfected lysate.Recommend Usage
Use it directly for immuno-precipitation, or heat lysate with SDS gel loading buffer to 95°C for 5 minutes followed by rapid cooling for western blot application. If dissociating conditions are required, add reducing agent prior to heating.
Storage Buffer
In modified RIPA Lysis Buffer.
Storage Instruction
Store at -80°C. Aliquot to avoid repeated freezing and thawing.
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Applications
Western Blot
Immunoprecipitation
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Gene Info — ZSCAN21
Entrez GeneID
7589GeneBank Accession#
BC047309Protein Accession#
AAH47309Gene Name
ZSCAN21
Gene Alias
DKFZp434L134, DKFZp686H10254, NY-REN-21, ZNF38, Zipro1
Gene Description
zinc finger and SCAN domain containing 21
Omim ID
601261Gene Ontology
HyperlinkOther Designations
zinc finger protein 38|zinc finger protein 38 (KOX 25)|zinc finger protein NY-REN-21 antigen
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