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Last updated: 2021/3/7

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COVID-19 Pseudovirus Neutralizing Antibody Assay (Luciferase) 

  • Catalog # : KA6152
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  • Specification
  • Product Description:
  • COVID-19 Pseudovirus Neutralizing Antibody Assay (Luciferase) provides a highly sensitive and quantitative measurement of neutralizing antibodies.
  • Storage Instruction:
  • Store at -80°C
    Aliquot to avoid repeated freezing and thawing.
  • Supplied Product:
  • Kit content:
    1 kit is sufficient for 100 assays in 24 well plates
    1. 293T-hACE2 cell, 2x106cells/vial, DMEM with 10% FBS
    2. SARS-CoV-2 Pseudovirus Expressing Luciferase
  • Materials Required But NOT Supplied:
  • 1. SARS-CoV-2 Monoclonal Antibody, Cat# MAB22854-M07, Abnova
    2. ZymeFree™ Enzyme Free Cell Dissociation Reagent, Cat# TCL028-100ML, HIMEDIA
    3. DMEM culture medium containing 10% FBS (complete medium)
    4. Luciferase Assay System, Cat# E1500, Promega
     a. Luciferase Assay Substrate (lyophilized)
     b. Luciferase Assay Buffer
     c. Luciferase Cell Culture Lysis Reagent, 5X
    5. TC-Treated 24 Well Plates, Cat# 3524, Corning
    6. OptiPlate™-96, Cat# 6005290, PerkinElmer
  • Suitable Sample:
  • 1. Immunized Serum
    2. Infected Serum
    3. Neutralizing Antibodies
    4. Peptide Inhibitors or Proteins
    5. Compounds Targeting Spike Induced Cell Fusion
  • Sample Volume:
  • 10-50 uL
  • Detection Method:
  • Luciferase Activity
  • Regulatory Status:
  • For research use only (RUO)
  • Applications
  • Enzyme Activity
  • Enzyme Activity
  • The luciferase activity of neutralizing antibodies using COVID-19 Pseudovirus Neutralizing Antibody Assay (Luciferase) (Cat# KA6152).
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