APRT (Human) ELISA Kit
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Specification
Product Description
APRT (Human) ELISA Kit is a competitive enzyme-linked immunosorbent assay for quantitative measurement of human APRT in plasma, serum, cell culture, and cell lysate.
Suitable Sample
plasma, serum, cell culture, and cell lysate
Sample Volume
50 uL
Label
Peroxidase-conjugated
Detection Method
Colorimetric
Assay Type
Quantitative
Calibration Range
0.075 to 4.8 ng/mL
Reactivity
Human
Regulatory Status
For research use only (RUO)
Quality Control Testing
Standard curve
The standard curve is for the purpose of illustration only and should not be used to calculate unknowns. A standard curve should be generated each time the assay is performed.
Storage Instruction
Store components of the kit at 4°C or -20°C as described in the protocol.
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Applications
Quantification
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Gene Info — APRT
Entrez GeneID
353Protein Accession#
P07741Gene Name
APRT
Gene Alias
AMP, DKFZp686D13177, MGC125856, MGC125857, MGC129961
Gene Description
adenine phosphoribosyltransferase
Omim ID
102600Gene Ontology
HyperlinkGene Summary
Adenine phosphoribosyltransferase belongs to the purine/pyrimidine phosphoribosyltransferase family. A conserved feature of this gene is the distribution of CpG dinucleotides. This enzyme catalyzes the formation of AMP and inorganic pyrophosphate from adenine and 5-phosphoribosyl-1-pyrophosphate (PRPP). It also produces adenine as a by-product of the polyamine biosynthesis pathway. A homozygous deficiency in this enzyme causes 2,8-dihydroxyadenine urolithiasis. Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq
Other Designations
AMP diphosphorylase|AMP pyrophosphorylase|adenine phosphoribosyltransferase, isoform a|transphosphoribosidase
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