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2-NBDG Glucose Uptake Assay Kit 

  • Catalog # : KA6077
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  • Specification
  • Product Description:
  • 2-NBDG Glucose Uptake Assay Kit provides a sensitive and non-radioactive assay for measuring glucose uptake in cultured cells. The fluorescence signal can be monitored by fluorescence microscope or flow cytometer with a 488 nm laser and 530/30 nm emission filter (FITC channel). This Assay Kit is the most robust tool for monitoring glucose transporters.
  • Storage Instruction:
  • Store the kit at -20°C and avoid from light.
  • Note:
  • Example Data Analysis and Figures.
  • Fluorescence images of 2-NBDG uptake in CHO-K1 cells using the 2-NBDG Glucose Uptake Assay Kit. CHO-K1 cells at 40,000 cells/well/100 uL were seeded overnight in a 96-well black wall/clear bottom plate. Cells were treated with 20 mM Glucose (B) or 100 uM Phloretin (C) at 37°C for 1 hour, then incubated with 100 uM 2-NBDG staining solution for 20 minutes. Untreated control cells were stained under the same conditions. The fluorescence signal was measured using a fluorescence microscope with FITC filter.


  • Suitable Sample:
  • Cultured Cells.
  • Detection Method:
  • Fluorimetric
  • Platform:
  • Instrument: Flow cytometer
    Excitation: 488 nm laser
    Emission: 530/30 nm filter
    Instrument specification(s): FITC channel

    Instrument: Fluorescence microscope
    Excitation: FITC filter
    Emission: FITC filter
    Recommended plate: Black wall/clear bottom
  • Regulatory Status:
  • For research use only (RUO)
  • Applications
  • Functional Study
  • Flow Cytometry
  • Flow Cytometry
  • Flow cytometry of 2-NBDG uptake in CHO-K1 cells using the 2-NBDG Glucose Uptake Assay Kit. CHO-K1 cells were treated with or without 100 uM Phloretin at 37°C for 1 hour, then incubated with 100 uM 2-NBDG staining solution for 20 minutes. To prepare adherent CHO-K1 cells for flow cytometry, EDTA was used to detach cells after staining. Fluorescence intensity was measured using ACEA NovoCyte flow cytometer in FITC channel.
  • Application Image
  • Functional Study
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