PPM1F (Human) ELISA Kit
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Specification
Product Description
PPM1F (Human) ELISA Kit is a sandwich enzyme-linked immunosorbent assay for quantitative detection of human PPM1F in serum, plasma and cell culture.
Suitable Sample
Serum, Plasma, Cell Culture
Sample Volume
50 uL
Label
Peroxidase-conjugated
Detection Method
Colorimetric
Assay Type
Quantitative
Calibration Range
1.56 to 400 ng/mL
Reactivity
Human
Regulatory Status
For research use only (RUO)
Quality Control Testing
Standard curve
The standard curve is for the purpose of illustration only and should not be used to calculate unknowns. A standard curve should be generated each time the assay is performed.
Storage Instruction
Store components of the kit at 4°C or -20°C as described in the protocol.
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Applications
Quantification
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Gene Info — PPM1F
Entrez GeneID
9647Protein Accession#
P49593Gene Name
PPM1F
Gene Alias
CaMKPase, FEM-2, KIAA0015, POPX2, hFEM-2
Gene Description
protein phosphatase 1F (PP2C domain containing)
Gene Ontology
HyperlinkGene Summary
The protein encoded by this gene is a member of the PP2C family of Ser/Thr protein phosphatases. PP2C family members are known to be negative regulators of cell stress response pathways. This phosphatase can interact with Rho guanine nucleotide exchange factors (PIX), and thus block the effects of p21-activated kinase 1 (PAK), a protein kinase mediating biological effects downstream of Rho GTPases. Calcium/calmodulin-dependent protein kinase II gamma (CAMK2G/CAMK-II) is found to be one of the substrates of this phosphatase. The overexpression of this phosphatase or CAMK2G has been shown to mediate caspase-dependent apoptosis. An alternatively spliced transcript variant has been identified, but its full-length nature has not been determined. [provided by RefSeq
Other Designations
Ca(2+)/calmodulin-dependent protein kinase phosphatase|CaM-kinase phosphatase|PP2C phosphatase|partner of PIX 2|protein phosphatase 1F
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