Multidrug Resistance Assay Kit (Fluorometric)
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MES-SA and MES-SA/Dx5 cells were plated at a density of 5 x 104 cells/well and grown overnight. The following day, cells were treated with either 25 ug/mL cyclosporin A (blue), 50 ug/mL verapamil (green), or untreated (red).
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Specification
Product Description
Multidrug Resistance Assay Kit (Fluorometric) is a fluorescent assay used to detect multidrug resistance.
Suitable Sample
Adherent cells, Suspension cells
Sample Volume
100 uL
Detection Method
Fluorometric
Excitation
485 nm
Emission
535 nm
Regulatory Status
For research use only (RUO)
Quality Control Testing
Cyclosporin A and verapamil increase the percentage of calcein retained within cells.
MES-SA and MES-SA/Dx5 cells were plated at a density of 5 x 104 cells/well and grown overnight. The following day, cells were treated with either 25 ug/mL cyclosporin A (blue), 50 ug/mL verapamil (green), or untreated (red).
Storage Instruction
Store the kit at -20°C and avoid from light.
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Applications
Functional Study
Flow Cytometry
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